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07-1462 Anti-Zyxin Antibody

Anti-Zyxin Antibody is an antibody against Zyxin for use in WB.

Anti-Zyxin Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeletal Signaling Gene Symbol: ESP-2, HED-2, Zyxin-2, zyxin UniProt Number: Q15942
07-1462
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-1462
DescriptionAnti-Zyxin Antibody
Background InformationZyxin, a cytosolic protein containing 3 LIM zinc-binding domains, associates with the actin cytoskeleton near the adhesion plaques. Zyxin localizes to stress fibers, cell-cell junctions and colocalizes with vinculin, paxillin and VASP at focal adhesions but not at the tips of lamellipodia. Zyxin is involved in the regulation of actin dynamics and is a likely component of the signal transduction pathway mediating adhesion-stimulated changes in gene expression. Zyxin displays an actin nucleating activity that is dependent on its amino terminal proline-rich motifs and independent of Arp2/3 complex. Zyxin appears to function as an adaptor or docking protein to organize protein complexes important for regulated actin cytoskeleton dynamics.
References
Product Information
FormatAffinity Purified
Control
  • NIH/3T3 cell lysate
PresentationPurified rabbit polyclonal in PBS containing 0.1% sodium azide.
Applications
ApplicationAnti-Zyxin Antibody is an antibody against Zyxin for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenSynthetic peptide corresponding to the N-terminus of human Zyxin.
EpitopeN-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes the N-terminus of Zyxin.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryFocal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.
Gene Symbol
  • ESP-2
  • HED-2
  • Zyxin-2
  • zyxin
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression By similarity.
SUBUNIT STRUCTURE: Interacts with HPV type 6 protein E6. Does not interact significantly with E6 proteins from HPV types 11, 16, or 18. Interacts, via the Pro-rich regions, with the EVH1 domains of ENAH, EVL and VASP. Interacts with the first LIM domain of TES.
SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm › cytoskeleton. Nucleus. Cell junction › focal adhesion. Note: Associates with the actin cytoskeleton near the adhesion plaques. Enters the nucleus in the presence of HESX1.
SEQUENCE SIMILARITIES: Belongs to the zyxin/ajuba family.
Contains 3 LIM zinc-binding domains.
Molecular WeightApprox. 85 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected Zyxin on 10 µg of NIH/3T3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-1462 04053252670176