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AB4340 Anti-Zscan4 Antibody

AB4340
100 µg  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB4340
DescriptionAnti-Zscan4 Antibody
Alternate Names
  • Zinc finger and SCAN domain containing protein 4C
Background InformationZinc finger and SCAN domain containing protein 4C (UniProt: Q80VJ6) is encoded by the Zscan4c (also known as Gm397) gene (Gene ID: 245109) in mouse. Zscan4 is an embryonic stem (ES) cell-specific transcription factor required to regulate pluripotency, facilitate telomere elongation, and defy cellular senescence. It is expressed during zygotic genome activation in mouse two-cell embryos. It protects embryos from DNA damage by stabilizing the B-DNA form of microsatellite repeat DNA. It binds to the TGCACAC motif in CA/TG microsatellite repeats and regulates telomere elongation to maintain genomic stability and acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE) in undifferentiated ES cells. Dysfunction of Zscan4 during development or in mESCs leads to implantation failure, telomere maintenance defects, and chromosomal rearrangements. (Ref.: Srinivasan, R., et al., (2020), Sci. Adv., 6, eaaz9115)
References
Product Information
FormatAffinity Purified
Control
  • Embryonic Stem Cell lysates
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Zscan4, Cat. No. AB4340, is a rabbit polyclonal antibody that detects Zscan4 and is tested for use in Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesTested Applications

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of mouse Zscan4. The immunogen sequence is conserved between Zcsan4 family proteins (Zscan4b, Zscan4C, Zscan4d and Zscan4f).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects proteins of Zscan4 family (Zscan4b, Zscan4C, Zscan4d and Zscan4f). It targets an epitope within 16 amino acids from the C-terminal half.
Species Reactivity
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Zscan4c
  • Gm397
Purification MethodAffinity Purfied
UniProt Number
Molecular WeightTarget molecular weight ~ 60 kDa observed; 60.0 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse embryonic stem cell (mESCs) lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Zscan4 in mouse embryonic stem cell (mESCs) lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB4340 04053252859526

Documentation

Anti-Zscan4 Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Zscan4 Antibody Certificates of Analysis

TitleLot Number
Anti-Zscan4 3082492
Anti-Zscan4 - Q2144996 Q2144996
Anti-Zscan4 - 2520373 2520373
Anti-Zscan4 - 3200630 3200630
Anti-Zscan4 - 3385852 3385852
Anti-Zscan4 - 3690683 3690683
Anti-Zscan4 - 3807973 3807973
Anti-Zscan4 - 3905048 3905048
Anti-Zscan4 - 3924044 3924044
Anti-Zscan4 - 4005693 4005693

References

Reference overviewPub Med ID
Zscan4 is regulated by PI3-kinase and DNA-damaging agents and directly interacts with the transcriptional repressors LSD1 and CtBP2 in mouse embryonic stem cells.
Storm, MP; Kumpfmueller, B; Bone, HK; Buchholz, M; Sanchez Ripoll, Y; Chaudhuri, JB; Niwa, H; Tosh, D; Welham, MJ
PloS one  9  e89821  2014

Show Abstract
24594919 24594919
Role of Tet proteins in enhancer activity and telomere elongation.
Lu, F; Liu, Y; Jiang, L; Yamaguchi, S; Zhang, Y
Genes & development  28  2103-19  2014

Show Abstract
25223896 25223896