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MAB4234-C Anti-Wilms' Tumor Antibody, NT clone 6F-H2, Ascites Free

This Anti-Wilms' Tumor Antibody, NT clone 6F-H2, Ascites Free is validated for use in Immunocytochemistry, Immunoprecipitation, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting for the detection of Wilms' tumor protein.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: WT1, DDS, FS, MEACHS, MESOM, NPHS4, WAGR UniProt Number: P19544
MAB4234-C
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MICC, IF, IH(P), IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB4234-C
DescriptionAnti-Wilms' Tumor Antibody, NT clone 6F-H2, Ascites Free
Alternate Names
  • Wilms tumor protein
  • WT33
Background InformationWilms tumor protein (UniProt P19544; also known as WT33) is encoded by the WT1 (also known as DDS, FS, MEACHS, MESOM, NPHS4, WAGR) gene (Gene ID 7490) in human. The Wilms’ tumor gene WT1 was originally identified in the childhood kidney cancer Wilms’ tumor. The N-terminal region of WT1 protein contains a proline-rich region (a.a. 27-83) involved in transcriptional regulation, self-association, and RNA recognition, while its C-terminal region contains four zinc fingers (a.a 323-347, 353-377, 383-405, 414-438) that mediate DNA and RNA binding. The zinc finger domain of WT1 can bind to GC-rich sequences, such as the EGR-1 consensus sequence (5’-GCG(T/G)GGGCG-3’), the WTE motif (5'-GCGTGGGAGT-3'), or (TCC)n motif. Many genes responsible for cell growth and apoptosis, such as Bcl-2, Bcl-xL, BFL1, and c-myc, have been identified as downstream targets of WT1. There are four major alternatively spliced WT1 isoforms resulting from splicing at either or both of exon 5 (17AA) and exon 9 (KTS). All four major WT1 isoforms are overexpressed in leukemia and solid tumors and play oncogenic roles such as inhibition of apoptosis, and promotion of cell proliferation, migration and invasion.
References
Product Information
FormatPurified
PresentationPurified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Wilms' Tumor Antibody, NT clone 6F-H2, Ascites Free is validated for use in Immunocytochemistry, Immunoprecipitation, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting for the detection of Wilms' tumor protein.
Key Applications
  • Immunocytochemistry
  • Immunofluorescence
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunohistochemistry Analysis: An 1:250 dilution from a representative lot detected Wilms tumor protein in human kidney tissue.

Immunoprecipitation Analysis: A representative lot co-immunoprecipitated CRE-binding protein/CBP together with Wilms tumor protein WT1 from the lysate of a T-SV40 immortalized human glomerular epithelial cell (HGEC) line (Drossopoulou, G.I., et al. (2009). Am. J. Physiol. Renal Physiol. 297(3):F594-F603).

Western Blotting Analysis: A representative lot detected Wilms tumor protein WT1 in the CRE-binding protein/CBP immunoprecipitate obtained from the lysate of a T-SV40 immortalized human glomerular epithelial cell (HGEC) line (Drossopoulou, G.I., et al. (2009). Am. J. Physiol. Renal Physiol. 297(3):F594-F603).

Western Blotting Analysis: A representative lot detected Wilms tumor protein WT1 in lysates from mouse E15.5 embryonic kidney and human melanoma cell lines A375, SK-MEL-28, and WM-266-4 (Wagner, N., et al. (2008). Pflugers Arch. Eur. J. Physiol. 455(5):839-847).

Immunocytochemistry Analysis: A representative lot immunostained the nucleus of methanol-fixed human melanoma A375 cells by fluorescent immunocytochemistry (Wagner, N., et al. (2008). Pflugers Arch. Eur. J. Physiol. 455(5):839-847).

Immunofluorescence Analysis: A representative lot immunostained the PCNA-positive nuclei of proliferating cells in formalin-fixed, paraffin-embedded human melanoma tissue sections by fluorescent immunohistochemistry (Wagner, N., et al. (2008). Pflugers Arch. Eur. J. Physiol. 455(5):839-847).

Immunohistochemistry Analysis: A representative lot immunostained glomeruli in formalin-fixed, paraffin-embedded normal human kidney and Wilms' tumor sections (Wagner, N., et al. (2008). Pediatr. Nephrol. 23(9):1445-1453).

Immunohistochemistry Analysis: A representative lot detected vascular WT1 expression in 95% of 113 paraffin-embedded tumour tissues of various types. In most cases, nuclear WT1 staining of endothelial cells was seen (Wagner, N., et al. (2008). Oncogene. 27(26):3662-3672).

Immunohistochemistry Analysis: A representative lot immunostained the nucleus of perifollicular fibroblasts at the hair follicle in formalin-fixed, paraffin-embedded normal human skin sections. Most common melanocytic nevi do not express WT1, whereas Spitz nevi and dysplastic nevi show cytoplasmic WT1 staining. (Wagner, N., et al. (2008). Pflugers Arch. Eur. J. Physiol. 455(5):839-847).
Biological Information
ImmunogenRecombinantt human WT-1 N-terminal fragment (a.a. 1-173).
EpitopeAmino acids 1-173.
Clone6F-H2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with all spliced isoforms other than isoforms 6 and 9 reported by UniProt (P19544). Reactivity toward isoforms 6 and 9 has not been determined.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • WT1
  • DDS
  • FS
  • MEACHS
  • MESOM
  • NPHS4
  • WAGR
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~52 kDa observed. 49.19 kDa (isoform 1), 47.20 kDa (isoform 2), 47.51 kDa (isoform 3), 48.87 kDa (isoform 4), 34.45 kDa (isoform 5), 56.88 kDa (isoform 6), 55.21 kDa (isoform 7), 33.09 kDa (isoform 8) calculated. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Wilms tumor protein in 10 µg of Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB4234-C 04055977331288

Documentation

Anti-Wilms' Tumor Antibody, NT clone 6F-H2, Ascites Free SDS

Title

Safety Data Sheet (SDS) 

Anti-Wilms' Tumor Antibody, NT clone 6F-H2, Ascites Free Certificates of Analysis

TitleLot Number
Anti-Wilms' Tumor, NT clone 6F-H2, -2799567 2799567
Anti-Wilms' Tumor, NT clone 6F-H2, -Q2653195 Q2653195
Anti-Wilms' Tumor, NT clone 6F-H2, Ascites Free - 3393259 3393259
Anti-Wilms' Tumor, NT clone 6F-H2, Ascites Free - 3423400 3423400
Anti-Wilms' Tumor, NT clone 6F-H2, Ascites Free - 3429951 3429951
Anti-Wilms' Tumor, NT clone 6F-H2, Ascites Free - 3433926 3433926
Anti-Wilms' Tumor, NT clone 6F-H2, Ascites Free - 3451390 3451390
Anti-Wilms' Tumor, NT clone 6F-H2, Ascites Free - 3704211 3704211
Anti-Wilms' Tumor, NT clone 6F-H2, Ascites Free - 3773105 3773105
Anti-Wilms' Tumor, NT clone 6F-H2, Ascites Free - 3819520 3819520
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