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AB5062P Anti-Vesicular GABA Transporter (VGAT) Antibody

AB5062P
50 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, M, H, MkELISA, IF, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5062P
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Vesicular GABA Transporter (VGAT) Antibody
Alternate Names
  • Vesicular Inhibitory Amino Acid Transporter
Background InformationVesicular GABA Transporter (unc-47) homologue is a 525 amino acid long protein belonging to the family of neurotransmitter transporter (1). VGAT functions in the uptake of GABA and glycine into synaptic vesicles. Since the protein is bound to synaptic vesicles, VGAT antibodies are excellent markers of presynaptic GABAergic and glycinergic neurons.
Product Information
FormatAffinity Purified
Control
  • Brain tissue, rat retina lysate
PresentationPurified rabbit polyclonal in buffer containing PBS with 0.1% BSA.
Quality LevelMQ100
Applications
ApplicationThis Anti-Vesicular GABA Transporter (VGAT) Antibody is validated for use in ELISA, IF, IH for the detection of Vesicular GABA Transporter (VGAT).
Key Applications
  • ELISA
  • Immunofluorescence
  • Immunohistochemistry
Application NotesImmunohistochemistry/Immunofluorescence:
2-20 µg/mL of a previous lot was used on paraformaldehyde fixed sections.

ELISA:
1:10,000 – 1:100,000 dilution of a previous lot was used on ELISA, using 50-100 ng of control peptide (Cat. No. AG379) per well.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenA 17 amino acid peptide sequence near the carboxy terminal region of rat VGAT (McIntire, S., 1997).
EpitopeC-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificitySpecific for Vesicular GABA Transporter (VGAT). Vesicular GABA Transporter (unc-47) homologue is a 525 amino acid long protein belonging to the family of neurotransmitter transporter. The immunogen peptide shows no significant homology with other known proteins.
Species Reactivity
  • Rat
  • Mouse
  • Human
  • Monkey
Species Reactivity NoteRat. Antibody cross-reactivity with various species is not known. The immunogen sequence is 100% conserved in mouse, human and monkey.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II.
Gene Symbol
  • SLC32A1
  • VIAAT
  • VGAT
  • bA122O1.1
  • hVIAAT
Purification MethodImmunoAffinity Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9H598 # Involved in the uptake of GABA and glycine into the synaptic vesicles.
SIZE: 525 amino acids; 57415 Da
SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane; Multi-pass membrane protein (By similarity).
TISSUE SPECIFICITY : Retina. Expressed throughout the horizontal cells or more specifically at the terminals.
SIMILARITY: SwissProt: Q9H598 ## Belongs to the amino acid/polyamine transporter 2 family.
Molecular Weight57 kDa
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20ºC from date of receipt.
Packaging Information
Material Size50 µg
Global Trade Item Number
Catalogue Number GTIN
AB5062P 04053252287381