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07-2130 Anti-Ubiquitin Antibody

07-2130
100 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RIP, WBRbPurifiedPolyclonal Antibody
Description
Catalogue Number07-2130
DescriptionAnti-Ubiquitin Antibody
Alternate Names
  • Polyubiquitin-C
  • Ubiquitin
Background InformationUbiquitin functions as a marker to indicate the fate of other proteins in the cell. It can be attached as a single molecule or as a polyubiquitin chain. Although ubiquitinated proteins are oftentimes tagged for degradation by the 20S proteosome, ubiquitination can also indicate alternative fates depending on the lysine residue that is involved in linking the ubiquitin chain to the target protein.Ubiquitin chains linked by Lys33 to kinase substrates such as AMP-activated protein kinases, (AMPKα1, BRSK1, MARK3, MARK4, and NUAK1), may activate or deactivate these proteins, supporting the role for uniquitin in regulating signaling.
Product Information
FormatPurified
Control
  • Serum starved HeLa cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Ubiquitin antibody is validated for use in IP & WB for the detection of the Ubiquitin protein.
Key Applications
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern Blot Analysis: 0.5 µg/mL from a representative lot detected Ubiquitin on 10 µg of HeLa cell lysate.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human Ubiquitin.
EpitopeN-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes Ubiquitin at the N-terminus
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteDemonstrated to react with Human, Mouse, and Rat. Broad species reactivity expected based on sequence similarity.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • UBC
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~8 kDa observed. The calculated molecular weight of this protein is 8 kDa, but has been observed at ~24 kDa due to tetramers. (Kvint, K., et al. (2008). Mol Cell.30(4):498-506.)
Product Usage Statements
Quality AssuranceEvaluated by Immunoprecipitation in serum starved HeLa cell lysate.

Immunoprecipitation Analysis: 10 µg of this antibody detected Ubiquitin on 5 µL of serum starved HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
07-2130 04053252440090