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MABN2621-100UG Anti-UNC119 homolog A/mRG4 Antibody, clone A2

Anti-UNC119 homolog A/mRG4, clone A2, Cat. No. MABN2621, is a mouse monoclonal antibody that detects UNC119 homolog A (mRG4) and is tested for use in Immunohistochemistry, Immunofluorescence, and Western Blotting.

MABN2621-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABN2621-100UG
DescriptionAnti-UNC119 homolog A/mRG4 Antibody, clone A2
Alternate Names
  • Protein unc-119 homolog A (UniProt: Q9Z2R6
  • also known as Retinal protein 4
  • mRG4
Background InformationProtein unc-119 homolog A (UniProt: Q9Z2R6; also known as Retinal protein 4, mRG4) is encoded by the Unc119 (also known as Unc119h) gene (Gene ID: 22248) in murine species. UNC119 is an adaptor protein that is involved in the development of the vertebrate nervous system. It is widely expressed among vertebrates and invertebrates and is predominantly localized in photoreceptor synapses in the outer plexiform layer of the retina. It recognizes the acylated N-terminus of the rod photoreceptor transducin -subunit (T ). Apart from its presence in the mammalian retina, it is also detected in leukocytes, T-cells, lung fibroblasts, the adrenal glands, cerebellum, and kidney. UC119 is reported to activate Src-type tyrosine kinases associated with the interleukin-5 receptor and the T-cell receptor by interacting with Src homology domains localized in its N-terminal half. It is also reported to stimulate the induction of -smooth muscle actin and myofibroblast differentiation by TGF-β in human lung fibroblasts. UNC119 can undergo phosphorylation at serine 37, 39, and 41 by casein kinase 2 and these phosphorylations suppresses its interaction with KLHL18 (Kelch Like Family Member 18) and downregulates its KLHL18-mediated degradation. (Ref.: Chaya, T., et al. (2019). EMBO J. 38(23); e101409; Zhang, H., et al. (2011). Nat. Neurosci. 14(7); 874-880; Vepachedu, R., et al. (2007). J. Immunol. 179(1); 682-690).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-UNC119 homolog A/mRG4, clone A2, Cat. No. MABN2621, is a mouse monoclonal antibody that detects UNC119 homolog A (mRG4) and is tested for use in Immunohistochemistry, Immunofluorescence, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunofluorescence
Application NotesTested Applications

Immunofluorescence Analysis: A representative lot detected UNC119 homolog A/mRG4 in Immunofluorescence applications (Haeseleer, F., et al. (2008). Invest Ophthalmol Vis Sci. 49(6):2366-75).

Western Blotting Analysis: A representative lot detected UNC119 homolog A/mRG4 in Western Blotting applications (Haeseleer, F., et al. (2008). Invest Ophthalmol Vis Sci. 49(6):2366-75).

Immunohistochemistry Applications: A representative lot detected UNC119 homolog A/mRG4 in Immunohistochemistry applications (Haeseleer, F., et al. (2008). Invest Ophthalmol Vis Sci.;49(6):2366-75).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged full-length recombinant mouse UNC119 homolog A (mRRG4).
EpitopeUnknown
CloneA2
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone A2 is a mouse monoclonal antibody that detects murine UNC119 Homolog A protein (mRG4).
IsotypeIgG1κ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Unc119
Purification MethodProtein G purified
UniProt Number
Molecular Weight~28 kDa observed; 27.01 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Mouse retina tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected UNC119 homolog A (mRG4) in Mouse retina tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2621-100UG 04065267327754