Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABN681 Anti-TrkA Antibody, clone 6B2

Anti-TrkA Antibody, clone 6B2 is a highly specific mouse monoclonal antibody, that targets TrkA & has been tested in western blotting, IHC & ICC.

Anti-TrkA Antibody, clone 6B2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: NTRK1, MTC, TRK, TRKA UniProt Number: P04629
MABN681
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWB, IHC, ICCMAscitesMonoclonal Antibody
Description
Catalogue NumberMABN681
DescriptionAnti-TrkA Antibody, clone 6B2
Alternate Names
  • High affinity nerve growth factor receptor
  • Neurotrophic tyrosine kinase receptor type 1
  • TRK1-transforming tyrosine kinase protein
  • Tropomyosin-related kinase A
  • Tyrosine kinase receptor
  • Tyrosine kinase receptor A
  • Trk-A
  • gp140trk
  • p140-TrkA
Background InformationTrkA, apparent molecular weight 140 kDa, is a high affinity nerve growth factor (NGF). The Trk proto-oncogene family contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkA binds to nerve growth factor (NGF) and autophosphorylates on tyrosine residues (Tyr490, Tyr674, Tyr675, Tyr751 and Tyr785) to activate multiple downstream effector proteins. Phosphorylation at Tyr490 is required for Shc association and subsequent activation of the Ras-MAP kinase-signaling cascade, which leads to activation of Elk-1-dependent gene transcription and neurite growth. Phosphorylations at Tyr674 and Tyr675 lie within the catalytic domain of TrkA tyrosine kinase and reflect Trk kinase activity. Additionally, phosphorylation at Tyr751 is required for PI3-kinase association and activation of the Akt signaling cascade.
References
Product Information
FormatAscites
Control
  • Human brain tissue lysate
PresentationMouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-TrkA Antibody, clone 6B2 is a highly specific mouse monoclonal antibody, that targets TrkA & has been tested in western blotting, IHC & ICC.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunocytochemistry
Application NotesImmunohistochemistry Analysis: A 1:100 dilution from a representative lot detected TrkA in human brain and rat cerebellum tissues.

Immunocytochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected TrkA in PC-12 cells.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenPurified recombinant extracellular fragment of TrkA expressed in HEK293 cells.
Clone6B2
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NTRK1
  • MTC
  • TRK
  • TRKA
Purification MethodUnpurified
UniProt Number
Molecular Weight~87 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 4 isoforms ranging between ~77 kDa and ~87 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected TrkA in 10 µg of human brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN681 04053252909269

Documentation

Anti-TrkA Antibody, clone 6B2 SDS

Title

Safety Data Sheet (SDS) 

Anti-TrkA Antibody, clone 6B2 Certificates of Analysis

TitleLot Number
Anti-TrkA, clone 6B2 -VP1811302 VP1811302
Anti-TrkA, clone 6B2 - VP1403121 VP1403121
Anti-TrkA, clone 6B2 - VP1405160 VP1405160
Anti-TrkA, clone 6B2 - VP1307025 VP1307025
Anti-TrkA, clone 6B2 - VP2106185 VP2106185
Anti-TrkA, clone 6B2 - VP2205190 VP2205190
Anti-TrkA, clone 6B2 - VP2301250 VP2301250
Anti-TrkA, clone 6B2 -VP1502256 VP1502256
Anti-TrkA, clone 6B2 -VP1511119 VP1511119
Anti-TrkA, clone 6B2 -VP1602117 VP1602117
Show more