Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABF1067-25UL Anti-Tapasin

Anti-Tapasin Antibody, Cat. No. ABF1067, is a rabbit polyclonal antibody that detects Tapasin in murine cells and has been tested for use in Immunoprecipitation and Western Blotting.

Anti-Tapasin MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: Tapbp, Tapa UniProt Number: Q9R233
ABF1067-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MIP, WBRbUnpurifiedPolyclonal Antibody
Description
Catalogue NumberABF1067-25UL
DescriptionAnti-Tapasin
Alternate Names
  • Tapasin
  • TPN
  • TPSN
  • TAP-associated protein
  • TAP-binding protein
Background InformationTapasin (UniProt: Q9R233; also known as TPN, TPSN, TAP-associated protein, TAP-binding protein) is encoded by the Tapbp (also known as Tapa) gene (Gene ID: 21356) in the murine species. Tapasin is a single-pass type I membrane glycoprotein protein that is involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide. It is synthesized with a signal peptide of 23 amino acids, which is subsequently cleaved to generate mature Tapasin. It assists in loading of antigenic peptides on to class I molecules in the endoplasmic reticulum (ER). It is also reported to support the binding of high-affinity peptides to MHC class I in conjunction with ERp57. Defects in Tapasin expression are linked to destabilization of the MHC class I loading complex and diminution in the expression of MHC molecules at the cell surface. Tapasin is reportedly down-regulated in several human carcinomas and its re-expression in Tapasin-deficient cancer cells restores the expression of MHC class I antigen complexes and augment cancer cell immunogenicity, which helps in longer survival in animals bearing metastatic tumors. (Ref.: Lou, Y et al. (2008). Clin Cancer Res. 14(5); 1494-1501).
References
Product Information
FormatUnpurified
PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
Applications
ApplicationAnti-Tapasin Antibody, Cat. No. ABF1067, is a rabbit polyclonal antibody that detects Tapasin in murine cells and has been tested for use in Immunoprecipitation and Western Blotting.
Key Applications
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunoprecipitation Analysis: A representative lot detected Tapasin in K42 cells (Lum, R., et. al. (2016). J Biol Chem. 291(37):19631-41) and L-cells and BW5147 thymoma cells (Suh, W.K., et. al. (1999). J Immunol. 162(3):1530-40).

Western Blotting Analysis: A representative lot detected Tapasin in CMT.64 cells treated with IFN- (Lou, Y., et. al. (2008). Clin Cancer Res. 14(5):1494-501).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of murine Tapasin.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Tapasin in murine cells. It targets an epitope within 20 amino acids from the C-terminal region.
IsotypeIgG
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypePolyclonal Antibody
Gene Symbol
  • Tapbp
  • Tapa
Purification MethodUnpurified
UniProt Number
Molecular Weight51 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Tapasin in 10 µg of NIH/3T3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABF1067-25UL 04054839480126