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MABC534 Anti-TPM3 Antibody, isoform TC22, clone TC22-4

MABC534
100 μg  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, ELISA, WB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC534
DescriptionAnti-TPM3 Antibody, isoform TC22, clone TC22-4
Alternate Names
  • Tropomyosin alpha-3 chain
  • Gamma-tropomyosin
  • Tropomyosin-3
  • Tropomyosin-5
  • hTM5
Background InformationTropomyosins are dimmers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. The human TPM3 gene (Gene ID 7170; also known as TM3, TM5, TRK, CFTD, NEM1, TM-5, TM30, CAPM1, TM30nm, TPMsk3, hscp30, HEL-189, HEL-S-82p, OK/SW-cl.5) encodes seven tropomyosin alpha-3 chain isoforms (UniProt P06753; also known as Tropomyosin-3) as a result of alternative splicing. Spliced variants 1, 2, 4, and 5 are also known as the skeletal muscle, TM30nm, TC22, and hTM5 isoform, respectively. The TC22 and the hTM5 variants are identical in sequence other than the 25 amino acids at their C-terminal end. TC22 is expressed in 100% of colorectal carcinomas, but is not expressed in normal colon epithelial cells. The anticarcinogenic potency of sulfasalazine (SASP) and its active metabolite 5-aminosalicylate (5-ASA, mesalamine) in protecting ulcerative colitis (UC) patients from developing colorectal cancer is directly linked to their efficacy in reducing cellular expression of TC22. Likewise, siRNA-mediated TC22 downregulation is shown to significantly alter several critical carcinogenic pathways in the human colon adenocarcinoma LS180 cells.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-TPM3 Antibody, isoform TC22, clone TC22-4 is validated for use in Western blotting, Immunohistochemistry, ELISA and FLow Cytometry for the detection of TPM3.
Key Applications
  • Flow Cytometry
  • ELISA
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern Blotting Analysis: A representative lot detected endogenous TC22 in human colorectal carcinoma T84 cell lysate, as well as recombinant human TC22, but not five other human TPM3 isoforms (Lin, J.L., et al. (2002). Gastroenterology. 123(1):152-162; Das, K.K., et al. (2009). Mol Pharmacol. 76(1):183-191).
ELISA Analysis: A representative lot specifically detected recombinant human TC22, but not five other human TPM3 isoforms (Das, K.K., et al. (2009). Mol Pharmacol. 76(1):183-191.).
Flow Cytometry Analysis: A representative lot detected time-dependent TC22 downregulation in LS180 cells upon 2 mM 5-Aminosalicylate (5-ASA) treatment (Das, K.K., et al. (2009). Mol Pharmacol. 76(1):183-191.)
Immunohistochemistry: Representative lots detected TC22 expression in human colon carcinoma and adenomatous polyp tissues, but not in normal colon tissue samples (Lin, J.L., et al. (2002). Gastroenterology. 123(1):152-162; Watari, J., et al. (2008). Clin Gastroenterol Hepatol. 6(4):409-17).
Biological Information
ImmunogenRecombinant human TC22 and boosts with TC22-specific C-terminal peptide.
EpitopeNear C-terminus
CloneTC22-4
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone TC22-4 selectively detects human tropomyosin alpha-3 chain (TMP3) spliced variant TC22 (isoform 4), but not spliced Isoform 1 (Skeletal muscle), 2 (TM30nm), 3, 5 (hTM5), 6, or 7.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TPM3
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~32 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in recombinant human TC22 protein.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected TPM3 in 10 µg of recombinant human TC22 protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC534 04055977181609

Documentation

Anti-TPM3 Antibody, isoform TC22, clone TC22-4 SDS

Title

Safety Data Sheet (SDS) 

Anti-TPM3 Antibody, isoform TC22, clone TC22-4 Certificates of Analysis

TitleLot Number
Anti-TPM3, isoform TC22, -Q2586387 Q2586387

References

Reference overviewPub Med ID
Mesalamine suppresses the expression of TC22, a novel tropomyosin isoform associated with colonic neoplasia.
Das, KK; Bajpai, M; Kong, Y; Liu, J; Geng, X; Das, KM
Molecular pharmacology  76  183-91  2009

Show Abstract
19369484 19369484
Effect of eradication of Helicobacter pylori on the histology and cellular phenotype of gastric intestinal metaplasia.
Watari, J; Das, KK; Amenta, PS; Tanabe, H; Tanaka, A; Geng, X; Lin, JJ; Kohgo, Y; Das, KM
Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association  6  409-17  2008

Show Abstract
18321787 18321787
Isolation and sequencing of a novel tropomyosin isoform preferentially associated with colon cancer.
Lin, JL; Geng, X; Bhattacharya, SD; Yu, JR; Reiter, RS; Sastri, B; Glazier, KD; Mirza, ZK; Wang, KK; Amenta, PS; Das, KM; Lin, JJ
Gastroenterology  123  152-62  2002

Show Abstract
12105844 12105844

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.