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MABS2217-25UG Anti-Syntaxin 5 Antibody, clone 1G11

Anti-Syntaxin 5, clone 1G11, Cat. No. MABS2217, is a mouse monoclonal antibody that detects Syntaxin 5 and is tested for use in Immunocytochemistry and Western Blotting.

MABS2217-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABS2217-25UG
DescriptionAnti-Syntaxin 5 Antibody, clone 1G11
Alternate Names
  • STX-5
  • Sed5
Background InformationSyntaxin-5 (UniProt: Q08851; also known as STX-5) is encoded by the Stx5 (also known as Stx5a) gene (Gene ID: 65134) in rat. Syntaxin-5 is a single-pass type IV membrane protein that localizes throughout the Golgi apparatus and is most abundant in the cis-most cisternae. It is found in the endoplasmic reticulum (ER)-Golgi intermediate compartment membrane. It has a cytoplasmic domain (aa 1-333), a transmembrane domain (aa 334-354), and a single amino acid vesicular domain (aa 355). Its expression has been reported in the brain, heart, spleen, lung, liver, kidney, and testis. Syntaxin-5 mediates ER to Golgi transport and together with vesicular transport factor p115 (USO1) and Golgi matrix protein GM130 (GOLGA2), it is involved in tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Syntaxin-5 is shown to protect cells against proteotoxicity and is involved in clearance of insoluble protein deposits. (Ref.: Babazadeh, R., et al. (2019). Cell Rep. 28(8); 2096-2110; Diao, A., et al (2008); J. Biol. Chem. 283(11); 6957-6967).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Syntaxin 5, clone 1G11, Cat. No. MABS2217, is a mouse monoclonal antibody that detects Syntaxin 5 and is tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: A representative lot detected Syntaxin-5 in Western Blotting applications (Williams, A.L., et. al. (2004). Mol Biol Cell. 15(1):162-75).

Immunocytochemistry Analysis: A representative lot detected Syntaxin-5 in Immunocytochemistry Blotting applications (Williams, A.L., et. al. (2004). Mol Biol Cell. 15(1):162-75).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 83 amino acids from the C-terminal half, SNARE motif.
EpitopeC-terminal half
Clone1G11
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 1G11 is a mouse monoclonal antibody that detects Syntaxin-5. It targets an epitope within the cytoplasmic domain in the N-terminal half. It detects both isoform 1 and 2.
IsotypeIgG1κ
Species Reactivity
  • Rat
Species Reactivity NoteRat. Predicted to react with Human, Mouse, Bovine based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Stx5
  • Stx5a
Purification MethodProtein G purified
UniProt Number
Molecular Weight~40 and ~34 kDa observed; 39.75 and 34.12 kDa, respectively for isoform 1 and 2. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Rat brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Syntaxin-5 in Rat brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS2217-25UG 04065265944717