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ABF1215-AF488 Anti-SLP76 Antibody, Alexa Fluor® 488 Conjugate

This rabbit polyclonal Anti-SLP76, Alexa Fluor® 488 Conjugate, Cat. No. ABF1215-AF488 is validated for use in Flow Cytometry for the detection of SLP76 (lymphocyte cytosolic protein 2).

ABF1215-AF488
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABF1215-AF488
DescriptionAnti-SLP76 Antibody, Alexa Fluor® 488 Conjugate
Alternate Names
  • Lymphocyte cytosolic protein 2
  • SH2 domain-containing leukocyte protein of 76 kDa
  • SLP-76 tyrosine phosphoprotein
  • SLP76
Background InformationLymphocyte cytosolic protein 2 (UniProt: Q13094; also known as Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein, SLP76) is encoded by the gene LCP2 (Gene ID 3937) in human. SLP-76 is a cytoplasmic adaptor phosphoprotein that contains both SH2 and SAM domains and is involved in B and T cell receptor signaling. Its amino terminus contains an acidic region that includes a PEST domain and several tyrosine residues that are phosphorylated following TCR ligation. SLP-76 also contains a central proline-rich domain and a SH2 domain at its carboxy terminal. SLP-76 can be phosphorylated on multiple tyrosine residues by the upstream kinases ZAP-70 and Lck. SLP-76 phosphorylation plays an important role in T cell-mediated IL-2 production by allowing phosphorylated Vav to bind and this complex stimulates NFAT and IL-2 gene activation after TCR engagement. SLP-76 is highly expressed in spleen, thymus, and peripheral blood leukocytes and T cells. It is expressed in B cell lines at lower levels and is not detected in fibroblast and neuroblastoma cell lines. SLP-76 is reported to play a positive role in promoting T cell development and activation as well as mast cell and platelet function. Overexpression of SLP-76 is shown to enhance IL-2 transcription after TCR signaling.
References
Product Information
FormatAlexaFluor®488
PresentationPurified rabbit polyclonal antibody Alexa Fluor® 488 conjugate in PBS with 1.5% BSA and 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis rabbit polyclonal Anti-SLP76, Alexa Fluor® 488 Conjugate, Cat. No. ABF1215-AF488 is validated for use in Flow Cytometry for the detection of SLP76 (lymphocyte cytosolic protein 2).
Key Applications
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: 1:400 dilution from a representative lot detected SLP76 in 4% paraformaldeyde-fixed, 0.2% Triton X-100-permeabilized THP-1 human monocytic cells.

The unconjugated antibody (Cat. No. ABF1215) is also available for Western blotting application.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human SLP76.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody Alexa Fluor® 488 conjugate detected SLP76 (lymphocyte cytosolic protein 2) in Jurkat cells by flow cytometry.
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Rhesus Macaque based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • LCP2
Purification MethodAffinity purified.
UniProt Number
Molecular Weight60.2 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in Jurkat cells.

Flow Cytometry Analysis: A 1:400 diluution of this antibody detected SLP76 in 4% paraformaldeyde-fixed, 0.2% Triton X-100-permeabilized Jurkat human T lymphocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABF1215-AF488 04054839134340

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