Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABE1337-100UG Anti-SETD6

Anti-SETD6, Cat. No. ABE1337, is a highly specific rabbit polyclonal antibody that targets N-lysine methyltransferase SETD6 and has been tested for use in Immunocytochemistry and Western Blotting.

Research Category: Epigenetics & Nuclear Function Gene Symbol: SETD6 UniProt Number: Q8TBK2
ABE1337-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1337-100UG
DescriptionAnti-SETD6
Alternate Names
  • N-lysine methyltransferase SETD6
  • SET domain-containing protein 6
Background InformationN-lysine methyltransferase SETD6 (UniProt: Q8TBK2; also known as SET domain-containing protein 6) is encoded by the SETD6 gene (Gene ID: 79918) in human. N-lysine methyltransferase SETD6 is a SET domain protein that specifically monomethylates lysine 310 of NF-kappa B-p65 that leads to down-regulation of NF-kappa B transcription factor activity. It can also monomethylate lysine 8 of H2AZ (H2AZK8me1). SETD6 is required for the maintenance of embryonic stem cell self-renewal. Primary urothelial cells and normal bladder tissue have almost undetectable SETD6 levels that are shown to increase in transformed urothelial cells and is further increased in bladder cancer cells and tissues. Overexpression of SETD6 in transformed urothelial cells increases their survival and colony formation. SETD6 is shown to interact with the oxidative stress sensor protein DJ1, which is required for Nrf2-dependent transcription of antioxidant target genes. Under basal conditions, SETD6 and DJ1 are associated at chromatin and SETD6 inhibits DJ1 activity, which leads to the repression of Nrf2-dependent transcription. However, under conditions of oxidative stress SETD6 mRNA and protein levels decline that leads to elevation in Nrf2 expression and diminish interaction between SETD6 and DJ1. Two isoforms of SETD6 have been described that are produced by alternative splicing. (Ref.: Mukherjee, N., et al. (2017). Oncotarget. 8(9); 15114-15125; Chen, A., et al. (2016). Biochim. Biophys. Acta. 1859(2):420-427).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-SETD6, Cat. No. ABE1337, is a highly specific rabbit polyclonal antibody that targets N-lysine methyltransferase SETD6 and has been tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SETD6 in HEK293 cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of human N-lysine methyltransferase SETD6, isoform 1.
EpitopeN-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects N-lysine methyltransferase SETD6 in human cells. it targets an epitope within 18 amino acids from the N-terminal region.
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SETD6
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~53 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human kidney tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected SETD6 in human kidney tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1337-100UG 04054839448034

Documentation