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MABF2215-100UL Anti-SARS-CoV S-Δ 10 fragment Antibody, clone 1A9

Anti-SARS-CoV S- D10 fragment, clone 1A9, Cat. No. MABF2215, is a mouse monoclonal antibody that detects SARS-CoV2 spike glycoprotein and is tested for use in ELISA, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Neutralizing, and WB.

MABF2215-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2215-100UL
DescriptionAnti-SARS-CoV S-Δ 10 fragment Antibody, clone 1A9
Alternate Names
  • S Glycoprotein
  • SARS-CoV Glycoprotein
Background InformationSARS-CoV-1/2 Spike glycoprotein (UniProt: P59594/P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S gene (Gene ID: 1489668/43740568) in SARS-CoV-1/2 virus. The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 protein, which is reported to be well conserved, is responsible for membrane fusion. (Ref.: Zheng Z., et al. (2020). Euro Surveill. 25(28); 2000291; Lip, K-M., et al. (2006). J. Virol. 80(2); 941-950).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
Quality LevelMQ200
Applications
ApplicationAnti-SARS-CoV S- D10 fragment, clone 1A9, Cat. No. MABF2215, is a mouse monoclonal antibody that detects SARS-CoV2 spike glycoprotein and is tested for use in ELISA, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Neutralizing, and WB.
Key Applications
  • ELISA
  • Flow Cytometry
  • Immunofluorescence
  • Immunoprecipitation
  • Neutralizing
  • Western Blotting
Application NotesELISA Analysis: A representative lot detected SARS-CoV Spike protein in ELISA applications (Ng, O.W., et. al. (2014). PLoS One. 9(7):e102415).

Western Blotting Analysis: A representative lot detected SARS-CoV Spike protein in Western Blotting applications (Lip, K.M., et. al. (2006). J Virol. 80(2):941-50; Ng, O.W., et. al. (2014). PLoS One. 9(7):e102415).

Immunoprecipitation Analysis: A representative lot immunoprecipitated SARS-CoV Spike protein in Immunoprecipitation applications (Ng, O.W., et. al. (2014). PLoS One. 9(7):e102415).

Immunofluorescence Analysis: A representative lot detected SARS-CoV Spike protein in Immunofluorescence applications (Lip, K.M., et. al. (2006). J Virol. 80(2):941-50).

Neutralizing: A representative lot partially neutralized SARS-CoV spike protein in Neutralizing applications (Lip, K.M., et. al. (2006). J Virol. 80(2):941-50; Ng, O.W., et. al. (2014). PLoS One. 9(7):e102415).

Flow Cytometry Analysis: A representative lot detected SARS-CoV Spike protein in Flow Cytometry applications (Lip, K.M., et. al. (2006). J Virol. 80(2):941-50).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenGST-tagged SARS-CoV Spike protein (SD 10 fragment).
EpitopeC-terminal
Clone1A9
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 1A9 is a mouse monoclonal antibody that detects SARS-CoV spike protein. It targets an epitope within 20 amino acids from the C-terminal, S2 subunit.
IsotypeIgG1κ
Species Reactivity
  • Virus
Species Reactivity NoteVirus.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • S
Purification MethodProtein G purified
UniProt Number
Molecular Weight~180 kDa observed; 139.12 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in SARS-CoV-2 Spike protein (ECD, His & FLAG® Tag).

Western Blotting Analysis: A 1:250 dilution of this antibody detected SARS-CoV-2 Spike protein (ECD, His & FLAG® Tag).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2215-100UL 04065265398077