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ABF1060-25UG Anti-SARS-CoV-2 ACE2 binding domain

Anti-SARS-CoV-2 ACE2 binding domain, Cat. No. ABF1060, is a rabbit polyclonal antibody that detects Spike protein of SARS-CoV-2 virus and is tested for use in Immunofluorescence and Western Blotting.

ABF1060-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABF1060-25UG
DescriptionAnti-SARS-CoV-2 ACE2 binding domain
Alternate Names
  • SARS-CoV-2 Spike glycoprotein
  • S glycoprotein
  • E2
  • Peplomer protein
Background InformationSARS-CoV-2 Spike glycoprotein (UniProt: P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S gene (Gene ID: 43740568) in SARS-CoV-2 virus. The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 protein, which is reported to be well conserved, is responsible for membrane fusion.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in PBS with 5 mM sodium azide with 50% glycerol.
Quality LevelMQ200
Applications
ApplicationAnti-SARS-CoV-2 ACE2 binding domain, Cat. No. ABF1060, is a rabbit polyclonal antibody that detects Spike protein of SARS-CoV-2 virus and is tested for use in Immunofluorescence and Western Blotting.
Key Applications
  • Western Blotting
  • Immunofluorescence
Application NotesWestern Blotting Analysis: A 1:2,000 dilution from a representative lot detected SARS-CoV2 ACE2 binding domain in lysate from HEK293 cells transfected with cDNA encoding the SARS-CoV-2 binding domain) or GFP-SARS-CoV-2-binding domain fusion protein (Courtesy of Gerry Shaw Labs)

Immunofluorescence Analysis: A 1:3,000 dilution from a representative lot detected SARS-CoV2 ACE2 binding domain in HEK293 cells transfected with cDNA encoding the SARS-CoV2 S-protein ACE2 binding domain (aa 308-541)(Courtesy of Gerry Shaw Labs)

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenRecombinant fragment corresponding to the receptor binding domain (ACE2) of SARS-CoV-2 virus spike protein.
EpitopeN-terminal half
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Spike protein of SARS-CoV-2 virus. It targets an epitope within the receptor binding domain (ACE2).
IsotypeIgG1
Species Reactivity
  • Virus
Species Reactivity NotePredicted to react with SARS-CoV Virus based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • S
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~165 kDa observed; 141.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with recombinant SARS-CoV-2 Spike protein (ECD).

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant SARS-CoV-2 Spike protein (ECD).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABF1060-25UG 04065265651516