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MABC1233-100UL Anti-Rae1 Antibody, clone 14G7.1

Anti-Rae1, clone 14G7.1, Cat. No. MABC1233, is a mouse monoclonal antibody that detects mRNA export factor (Rae1) and has been tested for use in Western Blotting.

Anti-Rae1 Antibody, clone 14G7.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: RAE1, MRNP41 UniProt Number: P78406
MABC1233-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1233-100UL
DescriptionAnti-Rae1 Antibody, clone 14G7.1
Alternate Names
  • mRNA export factor
  • Rae1 protein homolog
  • mRNA-associated protein mrnp 41
Background InformationmRNA export factor (UniProt: P78406; also known as Rae1 protein homolog, mRNA-associated protein mrnp 41) is encoded by the RAE1 (also known as MRNP41) gene (Gene ID: 8480) in human. mRNA export factor (UniProt: P78406; also known as RAE1 protein homolog, mRNA-associated protein mrnp 41) is encoded by the RAE1 (also known as MRNP41) gene (Gene ID: 8480) in human. Rae1 is a member of the superfamily of WD-repeat proteins that plays a role in mitotic bipolar spindle formation. It contains seven WD repeats. Rae1 is also reported to bind to a GLEBS-like NUP98 motif at the nuclear pore complex through multiple domains, including WD-repeats. Also, the COOH-Terminal non- WD-repeat extension of Rae1 Is shown to be essential for NUP98 binding. Rae1 plays a role in nucleocytoplasmic transport and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. It is recruited from interphase nuclei to spindle microtubules during mitosis. RAE1 also interacts with NUMA1 via N-terminal end of the coiled-coil domain and this interaction promotes spindle formation in mitosis. (Ref.: Pritchard, CEJ et al. (1999). J. cell Biol. 145(2); 237-254).
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Rae1, clone 14G7.1, Cat. No. MABC1233, is a mouse monoclonal antibody that detects mRNA export factor (Rae1) and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST/His-tagged recombinant fragment corresponding to 179 amino acids from the C-terminal half of human mRNA export factor (Rae1).
Clone14G7.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 14G7.1 specifically detects human mRNA export factor (Rae1). it targets an epitope with in the C-terminal half.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RAE1
  • MRNP41
Purification MethodProtein G purified
UniProt Number
Molecular Weight~40 kDa observed; 40.97 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rae1 in HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalogue Number GTIN
MABC1233-100UL 04054839519475