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MABN2438-25UG Anti-RPE65 Antibody, clone KPSA1

Anti-RPE65, clone KPSA1, Cat. No. MABN2438, is a mouse monoclonal antibody that detects RPE65 and is tested for use in ELISA, Immunohistochemistry, Immunoaffinity Purification, and Western Blotting.

MABN2438-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABN2438-25UG
DescriptionAnti-RPE65 Antibody, clone KPSA1
Alternate Names
  • Retinoid isomerohydrolase
  • EC:3.1.1.64
  • All-trans-retinyl-palmitate hydrolase
  • Lutein isomerase
  • Meso-zeaxanthin isomerase
  • Retinal pigment epithelium-specific 65 kDa protein
  • Retinol isomerase
Background InformationRetinoid isomerohydrolase (UniProt: Q16518; also known as EC:3.1.1.64, All-trans-retinyl-palmitate hydrolase, Lutein isomerase, Meso-zeaxanthin isomerase, Retinal pigment epithelium-specific 65 kDa protein, Retinol isomerase) is encoded by the RPE65 gene (Gene ID: 6121) in human. Retinol isomerase is a lipid-anchor protein that is attached to the membrane when palmitoylated and is present in soluble form when unpalmitoylated. It is shown to be critical for retinoid cycle and is involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. It catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol that is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore. It can also catalyze the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid. The soluble form is reported to binds vitamin A (all-trans-retinol), making it available for lecithin retinol acyltransferase (LRAT) processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for isomerohydrolase processing to all-cis-retinol. The soluble form can be regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. It undergoes light-dependent intracellular transport to become more concentrated in the central region of the retina pigment epithelium cells. Mutations in RPE65 gene are known to cause Retinitis pigmentosa that is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. (Ref.: Golczak, M., et al. (2010). J. Biol. Chem. 285(13); 9667-9682; Moiseyev, G., et al. (2005). Proc. Natl. Acad. Sci. USA. 102(35):12413-12418).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl and 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-RPE65, clone KPSA1, Cat. No. MABN2438, is a mouse monoclonal antibody that detects RPE65 and is tested for use in ELISA, Immunohistochemistry, Immunoaffinity Purification, and Western Blotting.
Key Applications
  • Western Blotting
  • ELISA
  • Immunohistochemistry
  • Immunoaffinity Purification
Application NotesTested applications

Western Blotting Analysis: A representative lot detected RPE65 in Western Blotting applications (Golczak, M., et al. (2010). J Biol Chem. 285(13):9667-9682; Banskota, S., et al. (2022). Cell. 185(2):250-265.e16).

Immunoaffinity Purification: A representative lot was used for purification of crossed-linked RPE65.(Golczak, M., et al. (2010). J Biol Chem. 285(13):9667-9682).

Immunohistochemistry Applications: A representative lot detected RPE65 in Immunohistochemistry applications (Amengual, J., et al. (2014). Hum Mol Genet. 23(20):5402-17).

ELISA Analysis: A representative lot detected RPE65 in ELISA applications (Golczak, M., et al. (2010). J Biol Chem. 285(13):9667-9682).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenRecombinant full-length Human RPE65 expressed in Sf9 cells.
EpitopeC-terminal
CloneKPSA1
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone KPSA1 is a mouse monoclonal antibody that detects RPE65. It targets an epitope within 23 amino acid from the C-terminal region.
IsotypeIgG1κ
Species Reactivity
  • Bovine
  • Mouse
  • Human
Species Reactivity NoteHuman, Bovine, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RPE65
Purification MethodProtein G purified
UniProt Number
Molecular Weight~62 kDa observed; 60.95 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Bovine retina microsomal preparation.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected RPE65 in Bovine retina microsomal preparation.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2438-25UG 04065270172570