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05-623-AF647 Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 647 Conjugate

This Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 647 Conjugate is validated for use in Immunocytochemistry for the detection of RNA polymerase II.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: POLR2A, POLR2 UniProt Number: P24928
05-623-AF647
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MICCMAlexaFluor®647Monoclonal Antibody
Description
Catalogue Number05-623-AF647
DescriptionAnti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 647 Conjugate
Alternate Names
  • DNA-directed RNA polymerase II subunit RPB1
  • RNA polymerase II subunit B1
  • DNA-directed RNA polymerase II subunit A
  • DNA-directed RNA polymerase III largest subunit
  • RNA-directed RNA polymerase II subunit RPB1
  • RNA polymerase II
Background InformationDNA-directed RNA polymerase II subunit RPB1 (EC 2.7.7.6; EC 2.7.7.48; UniProt P24928; also known as DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, RNA polymerase II subunit B1, RPBh1, RpIILS) is encoded by the POLR2A (also known as POLR2, POLRA, RPB1, RPO2, RPOL2) gene (Gene ID 5430) in human. RNA polymerase II (Pol II) is a 550 kDa, multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
Product Information
FormatAlexaFluor®647
PresentationPurified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 647 Conjugate is validated for use in Immunocytochemistry for the detection of RNA polymerase II.
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RNA polymerase II in A431 and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-623 & 05-623-Z) is shown to be suitable also for Western blotting, chromatin IP, ChIP-seq, and immunocytochemistry.
Biological Information
ImmunogenKLH-conjugated, synthetic peptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II.
Epitopecarboxyl-terminal domain of RNA polymerase II
CloneCTD4H8
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityThis antibody recognizes Phospho- and non-phospho-RNA polymerase II.
IsotypeIgG1
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Yeast, Rat based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • POLR2A
  • POLR2
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~210-220 kDa observed
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected RNA polymerase II in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalogue Number GTIN
05-623-AF647 04055977298918