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MABC1690H Anti-RAINBOW Antibody, HRP conjugated

Anti-RAINBOW, HRP-conjugated, Cat. No. MABC1690H, is a mouse monoclonal antibody that recognizes four different Remazol dye-conjugated proteins and can be used to visualize prestained molecular weight markers in Western Blotting.

Anti-RAINBOW Antibody, HRP conjugated MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Secondary & Control Antibodies
MABC1690H
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AWBMHRPMonoclonal Antibody
Description
Catalogue NumberMABC1690H
DescriptionAnti-RAINBOW Antibody, HRP conjugated
Background InformationPre-stained proteins are commonly used as molecular weight markers in electrophoresis. Several protein markers are available as pre-stained proteins with vinyl sulfone dyes. After separation through electrophoresis, the protein of interest is visualized by specific antibody coupled to a fluorophore or by chemiluminescence detection system. Anti-RAINBOW antibody is a unique antibody that can detect pre-stained molecular markers from different vendors without the need to perform a manual marking and estimating molecular weights. Anti-RAINBOW antibody detects all marker proteins in commercially available RAINBOW markers tested and lacks cross-reactivity with unstained proteins in whole cell lysates of a variety of species. It displays equal detection of Remazol-dye stained proteins: phosphorylase b (red), BSA and ADH (blue), chymotrypsin (yellow) and lysozyme (orange).
References
Product Information
FormatHRP
PresentationPurified mouse monoclonal antibody IgG1 in PBS with StabilZyme® HRP Conjugate Stabilizer and 0.05% Kathon.
Quality LevelMQ100
Applications
ApplicationAnti-RAINBOW, HRP-conjugated, Cat. No. MABC1690H, is a mouse monoclonal antibody that recognizes four different Remazol dye-conjugated proteins and can be used to visualize prestained molecular weight markers in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected Remzaol-dye prestained protein standards in Western Blotting applications (Schuchner, S., et. al. (2016). Sci Rep. 6:31363).

Western Blotting Analysis: A 1:500 and 1:1,000 dilution from a representative lot detected protein standard.
Biological Information
ImmunogenPurified Remazol Brilliant Blue R (RBBR)-BSA, followed by RO16-Chymotrypsin, RGY-Catalase and RF3B-Lysozyme.
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityAnti-RAINBOW, HRP-conjugated is a mouse monoclonal antibody that can be used as a universal tool for visualizing prestained protein molecular weight markers in Western blot analysis. It recognizes and cross-reacts with comparable affinity with four different Remazol dye immunogens.
IsotypeIgG1κ
Species Reactivity
  • All
Species Reactivity NoteAll.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G purified
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Blue Pre-Stained Protein Standard.

Western Blotting Analysis: A 1:10,000; 1:20,000; 1:40,000; 1:80,000 dilution of this antibody detected Remzaol-dye prestained protein standards.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1690H 04054839402104