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MABT1574-25UG Anti-Profilin IIa Antibody, clone PF2A1-8F8

Anti-Profilin IIa, clone PF2A1-8F8, Cat. No. MABT1574, is a mouse monoclonal antibody that detects Profilin IIa and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

MABT1574-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABT1574-25UG
DescriptionAnti-Profilin IIa Antibody, clone PF2A1-8F8
Alternate Names
  • Profilin II
Background InformationProfilin-2 (UniProt: P35080; also known as Profilin II) is encoded by the PFN2 gene (Gene ID: 5217) in human. Profilins are actin-binding proteins that serve as key regulators of actin polymerization in cells. Five profilin isoforms have been identified in mammals: Profilins I, IIa, IIb, III and IV. Profilin binds to G-actin in a 1:1 complex and thereby sequesters monomeric actin. Profilin I is highly expressed in all tissues except skeletal muscle, heart, and brain, while profilin II is highly expressed in brain, skeletal muscle, and kidney. Profilin IIa is highly expressed in developing and adult neurons and is involved in correct neurite outgrowth. Profilin IIb appears to be a minor form and it differs from IIa in 13 amino acids in the C-terminal region. Knockdown of both profilins I and II is reported to inhibit neurite outgrowth, possibly impairing actin dynamics by reducing the availability of profilin -G-actin complexes for microfilament growth. In addition to its role in actin polymerization and neurite outgrowth, profilin IIa may also be involved in regulation of synaptic vesicles. It exhibits high affinity for proline-rich peptides and is shown to interact with survival of motor neurons protein (SMN). Mutated forms of SMN (Y272G and G279V) are reported to significantly reduce its interaction with profilin IIa. (Ref.: Sharma, A., et al. (2005). Exp. Cell Res. 305; 195-197; Witke, W., et al. (1998). EMBO J. 17; 967-976).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Profilin IIa, clone PF2A1-8F8, Cat. No. MABT1574, is a mouse monoclonal antibody that detects Profilin IIa and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunofluorescence
Application NotesTested Applications

Immunoprecipitation Analysis: A representative lot immunoprecipitated Profilin IIa in Immunoprecipitation applications (Sharma, A., et al. (2005). Exp Cell Res. 309(1): 185-97).

Immunofluorescence Analysis: A representative lot detected Profilin IIa in Immunofluorescence applications (Sharma, A., et al. (2005). Exp Cell Res. 309(1): 185-97).

Western Blotting Analysis: A representative lot detected Profilin IIa in Western Blotting applications (Sharma, A., et al. (2005). Exp Cell Res. 309(1): 185-97).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenT7-tagged full-length recombinant human Profilin IIa.
EpitopeC-terminal half
ClonePF2A1-8F8
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone PF2A1-8F8 is a mouse monoclonal antibody that specifically detects Profilin-II. It targets an epitope within 10 amino acids from the C-terminal half.
IsotypeIgG2a
Species Reactivity
  • Human
  • Rat
Species Reactivity NoteHuman, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PFN2
Purification MethodProtein G purified
UniProt Number
Molecular Weight~15 kDa observed; 15.05 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Human cerebral cortex tissue lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Profilin IIa in Human cerebral cortex tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT1574-25UG 04065268364604