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MABT1586-25UG Anti-Profilin 1 Antibody, clone 2C5

Anti-Profilin 1, clone 2C5, Cat. No. MABT1586, is a mouse monoclonal antibody that detects Profilin 1 and is tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.

MABT1586-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABT1586-25UG
DescriptionAnti-Profilin 1 Antibody, clone 2C5
Alternate Names
  • Profilin-1
Background InformationProfilin-1 (UniProt: P62962; also known as Profilin I) is encoded by the Pfn1 gene (Gene ID: 18643) in murine species. Five profilin isoforms have been identified in mammals: Profilins I, IIa, IIb, III and IV. They regulate actin dynamics at plasma membranes during the assembly, maintain and disassemble actin networks at the leading edge of locomoting animal cells during cytokinesis, and are involved in embryonic development and morphogenesis. Profilins bind to G-actin in a 1:1 complex and thereby sequesters monomeric actin. Profilin I is highly expressed in all tissues. It binds to actin and affects the structure of the cytoskeleton. In neurons, proper profilin 1 function is essential during development and is implicated in the formation and maintenance of the neuronal cytoskeleton, growth-cone formation, synaptogenesis, synaptic activities, shape and morphological dynamics, and the growth of dendrites and axons. Knockdown of both profilins I and II is reported to inhibit neurite outgrowth, possibly impairing actin dynamics by reducing the availability of profilin-G-actin complexes for microfilament growth. Higher levels of profilin 1 have been reported in subjects with severe aplastic anemia. G118V and C71G mutations in profilin 1 have been linked to Amyotrophic lateral sclerosis (ALS). (Ref.: Yu, H., et al. (2021). Front. Immunol. 12; 631954; Kaiei, M., et al. (2018). Sci. Rep. 8; Article 13102; Murk, K., et al. (2012). PLoS One. 7(3); e34167; Jockusch, BM., et al. (2007). Rev. Physiol. Biochem. Pharmacol. 159; 131-149).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Profilin 1, clone 2C5, Cat. No. MABT1586, is a mouse monoclonal antibody that detects Profilin 1 and is tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunofluorescence
Application NotesTested Applications

Immunocytochemistry Analysis: A representative lot detected Profilin-1 in Immunocytochemistry applications (Murk, K., et al. (2012). PLoS One. 7(3); e34167).

Western Blotting Analysis: A representative lot detected Profilin-1 in Western Blotting applications (Murk, K., et al. (2012). PLoS One. 7(3); e34167).

Immunofluorescence Analysis: A representative lot detected Profilin-1 in Immunofluorescence applications (Murk, K., et al. (2012). PLoS One. 7(3); e34167).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenA linear peptide corresponding to 14 amino acids from the N-terminal region of mouse Profilin-1.
EpitopeN-terminal
Clone2C5
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 2C5 is a mouse monoclonal antibody that detects Profilin 1. It targets an epitope within 14 amino acids from the N-terminal region.
IsotypeIgG1κ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Pfn1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~15 kDa observed; 14.96 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Mouse spleen tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Profilin 1 in Mouse spleen tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT1586-25UG 04065268529171