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ABT257 Sigma-AldrichAnti-Pro-Collagen Type I, A1/COL1A1

Anti-Pro-Collagen Type I Antibody is an antibody against Pro-Collagen Type I for use in Western Blotting, Flow Cytometry, Immunohistochemistry.

Anti-Pro-Collagen Type I, A1/COL1A1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
Technical Information

Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: COL1A1 UniProt Number: P02452

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	WB, FC, IHC	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	ABT257
Description	Anti-Pro-Collagen Type I, A1/COL1A1
Alternate Names	Pro-collagen alpha-1(I) chain Pro-Alpha-1 type I collagen
Background Information	Collagen alpha-1(I) chain (UniProt P02452; also known as Alpha-1 type I collagen) is encoded by the COL1A1 gene (Gene ID 1277) in human. Collagen is the major component of the extracellular matrix (ECM) and form the fibrils of tendons, ligaments, and bones. Type I collagen consists of two alpha I chains and one alpha 2 chain. Alpha-1 type I collagen is initially produced as an 1464-amino acid prepro-form with a signal peptide sequence (a.a. 1-22) and two propeptide sequences (a.a. 23-161 and a.a. 1219 –1464), the removal of which yields the mature alpha-1(I) chain. The mature alpha-1(I) chain is composed mostly of a large triple-helical region (a.a. 179-1192) sandwiched between two nonhelical segments known as the N-terminal telopeptide (a.a. 162-178; numbering based on the prepro-form) and the C-terminal telopeptide (a.a. 1193-1218; numbering based on the prepro-form). Collagen can be extracted from tissue via either enzymatic or non-enzymatic means. Collagen extracted using the proteolytic enzyme pepsin corresponds to the large triple-helical region, referred to as atelocollagen because both the N- and C-terminal telopeptides have been cleaved off by pepsin. On the other hand, collagen preparations obtained with non-enzymatic means (e.g. by acid extraction) have the intact telopeptides at both ends.

References

Product Information
Format	Affinity Purified
Presentation	Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-Pro-Collagen Type I Antibody is an antibody against Pro-Collagen Type I for use in Western Blotting, Flow Cytometry, Immunohistochemistry.
Key Applications	Western Blotting Flow Cytometry Immunohistochemistry
Application Notes	Western Blotting Analysis: A representative lot detected Pro-Collagen Type I, A1/COL1A1 in mouse lung and skin tissue lysate. Flow Cytometry Analysis: A representative lot detected Pro-Collagen Type I, A1/COL1A1 in mouse lung tissue. Immunohistochemistry Analysis: A representative lot detected Pro-Collagen Type I, A1/COL1A1 in mouse lung tissue.

Biological Information
Immunogen	Linear peptide corresponding to sequence derived from human Pro-Collagen Type I, A1/COL1A1 C-terminal propeptide region.
Epitope	C-terminal propeptide region
Concentration	Please refer to lot specific datasheet.
Host	Rabbit
Specificity	Cat. No. ABT257 Anti-Pro-Collagen Type I, A1/COL1A1 was raised against a sequence derived from the C-terminal propeptide (not the N-terminal propeptide) region. This polyclonal antibody will detect prepro- and pro-, but not mature collagen alpha-1(I) chain.
Species Reactivity	Human Mouse
Species Reactivity Note	Human, Mouse. Predicted to react with a broad range of species based on high sequence homology, including mammalian, avian, fish, and amphibian species.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_000079
Gene Symbol	COL1A1
Purification Method	Affinity Purfied
UniProt Number	P02452
Molecular Weight	~140 kDa observed

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in mouse skin tissue lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected Pro-Collagen Type I, A1/COL1A1 in 10 µg of mouse skin tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
ABT257	04055977136678

Documentation

Anti-Pro-Collagen Type I, A1/COL1A1 SDS

Title
Safety Data Sheet (SDS)

Anti-Pro-Collagen Type I, A1/COL1A1 Certificates of Analysis

Title	Lot Number
Anti-Pro-Collagen Type I, A1/COL1A1	2902699
Anti-Pro-Collagen Type I, A1/COL1A1 - 3185049	3185049
Anti-Pro-Collagen Type I, A1/COL1A1 - 3201707	3201707
Anti-Pro-Collagen Type I, A1/COL1A1 - 3225879	3225879
Anti-Pro-Collagen Type I, A1/COL1A1 - 3268885	3268885
Anti-Pro-Collagen Type I, A1/COL1A1 - 3275493	3275493
Anti-Pro-Collagen Type I, A1/COL1A1 - 3389910	3389910
Anti-Pro-Collagen Type I, A1/COL1A1 - 3596724	3596724
Anti-Pro-Collagen Type I, A1/COL1A1 - 3729946	3729946
Anti-Pro-Collagen Type I, A1/COL1A1 - 3777980	3777980

Technical Info

Title
White Paper: Further considerations of antibody validation and usage.

Related Products & Applications

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AB765P	Anti-Mouse Collagen Type I Antibody
ABT123	Anti-Collagen Type I Antibody
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