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MABE2019-25UL Anti-PrimPol Antibody, clone JUAM71D

Anti-PrimPol, clone JUAM71D , Cat. No. MABE2019, is a rat monoclonal antibody that detects DNA-directed primase/polymerase (PrimPol) and is tested for use in Immunofluorescence and Western Blotting.

MABE2019-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABE2019-25UL
DescriptionAnti-PrimPol Antibody, clone JUAM71D
Alternate Names
  • DNA-directed primase/polymerase protein
  • hPrimpol1
  • Coiled-coil domain-containing protein 111
Background InformationDNA-directed primase/polymerase protein (UniProt: Q96LW4; also known as hPrimpol1, Coiled-coil domain-containing protein 111) is encoded by the PRIMPOL (also known as CCDC111) gene (Gene ID: 201973) in human. PrimPol is an enzyme with both DNA primase and DNA polymerase activities and is involved in DNA damage tolerance. It is detected in nuclei and cytosol with a large fraction localized inside the mitochondria. Upon UV irradiation, it is rapidly redirected to chromatin to reprime DNA synthesis. It associates with nuclear chromatin during the G1 and S phases of unperturbed cell cycles. It contains two RPA1-binding motifs (RBM) (aa 513-527 and 548-556) of which RPA1 is shown to be essential for recruitment to chromatin. It binds to the basic cleft of RPA1. The RPA2-binding motif plays a supporting role in RPA1-binding. PrimPol is required to facilitate mitochondrial and nuclear replication fork progression by initiating de novo DNA synthesis using dNTPs and acting as an error-prone DNA polymerase and is able to bypass certain DNA lesions. It displays high capacity to tolerate DNA damage lesions and is able to synthesize DNA primers downstream of lesions and allows DNA replication to continue. It is also shown to realign primers ahead of 'unreadable lesions' thereby skipping the lesion. In vitro, PrimPol is reported to act as a DNA primase, elongating primers that PrimPol itself synthesizes, or as translesion synthesis DNA polymerase that elongates pre-existing primers across lesions. In murine models PrimPol knockout cells display hypersensitivity to DNA interstrand crosslinks-inducing drugs. Two isoforms of PrimPol have been described that are produced by alternative splicing. (Ref.: Diaz-Talavera, I., et al. (2022). Biomolecules. 12(2); 248; Gonzales-Acosta, D., et al. (2021). EMBO J. 40(14); e106355; Guilliam, TA., et al. (2017). Nat. Commun. 8:15222; Garcia-Gomez, S., et al. (2013). Mol. Cell. 52(4); 541-553; Mouron, S., et al. (2013). Nat. Struct. Mol. Biol. 20(12); 1383-1389).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-PrimPol, clone JUAM71D , Cat. No. MABE2019, is a rat monoclonal antibody that detects DNA-directed primase/polymerase (PrimPol) and is tested for use in Immunofluorescence and Western Blotting.
Key Applications
  • Western Blotting
  • Immunofluorescence
Application NotesTested applications

Western Blotting Analysis: A representative lot detected PrimPol in Western Blotting applications (Gonzalez-Acosta, D., et al. (2021). EMBO J ;40(14); e106355).

Immunofluorescence Analysis: A representative lot detected PrimPol in Immunofluorescence applications (Gonzalez-Acosta, D., et al. (2021). EMBO J. 40(14); e106355).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenHis-tagged, full-length, recombinant human DNA-directed primase/polymerase (PrimPol).
EpitopeUnknown
CloneJUAM71D
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone JUAM71D is a rat monoclonal antibody that detects DNA-directed primase/polymerase (PrimPol).
IsotypeIgG2b
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PRIMPOL
Purification MethodProtein G purified
UniProt Number
Molecular Weight~64 kDa observed; 64.61 kDa calculated Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected PrimPol in HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE2019-25UL 04065269800170