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MAB2226 Anti-Piccolo Antibody, clone 4G3.3

This Anti-Piccolo Antibody, clone 4G3.3 is validated for use in WB, IH(P) for the detection of Piccolo.

Anti-Piccolo Antibody, clone 4G3.3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Synapse & Synaptic Biology Gene Symbol: ACZ , Aczonin, DKFZp779G1236, KIAA0559 UniProt Number: Q9Y6V0
MAB2226
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, RWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB2226
DescriptionAnti-Piccolo Antibody, clone 4G3.3
Alternate Names
  • presynaptic cytomatrix protein
  • Aczonin
Background InformationPiccolo (presynaptic cytomatrix protein or PCLO) may act as a scaffolding protein involved in the organization of synaptic active zones. Synaptic vesicles dock and fuse in the active zone of the plasma membrane at chemical synapses. The presynaptic cytoskeletal matrix (PCM), which is associated with the active zone and is situated between synaptic vesicles, is thought to be involved in maintaining the neurotransmitter release site in register with the postsynaptic reception apparatus. The cycling of synaptic vesicles is a multistep process involving a number of proteins. Among the components of the PCM that orchestrate these events are Bassoon (BSN), RIM (RBBP8), Oboe, and Piccolo (PCLO) [supplied by ENTREZGENE].
References
Product Information
FormatPurified
Control
  • Rat brain lysate, mouse cerebellum
PresentationPurified mouse monoclonal IgMκ in buffer containing PBS and 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Piccolo Antibody, clone 4G3.3 is validated for use in WB, IH(P) for the detection of Piccolo.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry (Paraffin):
Representative images from a previous lot.
Piccolo staining pattern/morphology on mouse brain tissue/cerebellum. Tissue was pretreated with citrate pH 6.0, antigen retrieval. A previous lot of the antibody was diluted to 1:300, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is localized to the synaptic junction of neurons in the granular layer and Purkinje cells(arrow).
Biological Information
ImmunogenGST-tagged recombinant protein
Clone4G3.3
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes piccolo.
IsotypeIgMκ
Species Reactivity
  • Mouse
  • Rat
Species Reactivity NoteMouse and Rat.
Predicted to react with human based on 82% sequence homology and rat based on 92% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ACZ
  • Aczonin
  • DKFZp779G1236
  • KIAA0559
UniProt Number
UniProt SummaryFUNCTION: May act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking (By similarity).
SUBUNIT: Interacts with Rabac1/Pra1, RIMS2 and profilin (By similarity).
SUBCELLULAR LOCATION: Cell junction, synapse (By similarity). Note=Concentrated at the presynaptic side of synaptic junctions (By similarity).
ALTERNATIVE PRODUCTS: 3 named isoforms
DOMAIN: C2 domain 1 is involved in binding calcium and phospholipids. Calcium binds with low affinity but with high specificity and induces a large conformational change.
SIMILARITY: Contains 2 C2 domains.
SIMILARITY: Contains 1 PDZ (DHR) domain.
SEQUENCE CAUTION:
Sequence=AAD21789.2; Type=Erroneous gene model prediction;
Molecular Weight547 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by western blot on rat brain lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB2226 04053252727429

Documentation

Anti-Piccolo Antibody, clone 4G3.3 SDS

Title

Safety Data Sheet (SDS) 

Anti-Piccolo Antibody, clone 4G3.3 Certificates of Analysis

TitleLot Number
Anti-Piccolo, Clone 4G3.3 - 4103094 4103094
Anti-Piccolo, clone 4G3.3 - 2197285 2197285
Anti-Piccolo, clone 4G3.3 - 2547196 2547196
Anti-Piccolo, clone 4G3.3 - 3216025 3216025
Anti-Piccolo, clone 4G3.3 - 3752785 3752785
Anti-Piccolo, clone 4G3.3 - NG1545023 NG1545023
Anti-Piccolo, clone 4G3.3 - NG1865038 NG1865038
Anti-Piccolo, clone 4G3.3 -2607608 2607608
Anti-Piccolo, clone 4G3.3 -2700988 2700988
Anti-Piccolo, clone 4G3.3 Monoclonal Antibody 2955323
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References

Reference overviewPub Med ID
IQ-ArfGEF/BRAG1 is associated with synaptic ribbons in the mouse retina.
Osamu Katsumata,Naoki Ohara,Hideaki Tamaki,Tomoko Niimura,Hideaki Naganuma,Masahiko Watanabe,Hiroyuki Sakagami
The European journal of neuroscience  30  2009

Show Abstract 		19811534 19811534