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MABS1963-25UG Anti-PEG Antibody, clone AGP3

Anti-PEG, clone AGP3, Cat. No. MABS1963, is a mouse monoclonal antibody that detects Polyethylene glycol (PEG) and has been tested for use in ELISA, Immunohistochemistry, and Western Blotting.

MABS1963-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Description
Catalogue NumberMABS1963-25UG
DescriptionAnti-PEG Antibody, clone AGP3
Alternate Names
  • Polyethylene Glycol
Background InformationPolyethylene glycols (PEG) are non-toxic, uncharged, hydrophilic long chain polymers with a big exclusion volume in aqueous solution. The covalent attachment of PEG is commonly employed to modify proteins and drugs that can increase their bioavailability and reduce immunogenicity and proteolytic degradation. Clone AGP3 is a mouse monoclonal IgM antibody that displays high affinity to both short and long PEG molecules. It displays specificity for the repeating subunits present in PEG and can bind a broad range of PEG molecules. It is suitable for use as a capture antibody in ELISA with biotin-conjugated anti-PEG, clone 6.3 (Cat. No. MABS1966) as a detection reagent. Use of CHAPS detergent instead of Tween-20 is highly recommended in all washing buffers.
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgM in PBS with 0.04% sodium azide.
Applications
ApplicationAnti-PEG, clone AGP3, Cat. No. MABS1963, is a mouse monoclonal antibody that detects Polyethylene glycol (PEG) and has been tested for use in ELISA, Immunohistochemistry, and Western Blotting.
Key Applications
  • ELISA
  • Immunohistochemistry
  • Western Blotting
Application NotesWestern Blotting Analysis: A represenative lot detected PEG in Western Blotting applications (Cheng, T.L., et. al. (2005). Bioconjug Chem. 16(5):1225-31).

ELISA Analysis: A represenative lot detected PEG in ELISA applications (Cheng, T.L., et. al. (2005). Bioconjug Chem. 16(5):1225-31).

Immunohistochemistry Analysis: A represenative lot detected PEG in Immunohistochemistry applications (Ton, N.C., et. al. (2007). Clin Cancer Res. 13(23):7113-8).
Biological Information
ImmunogenPEG-conjugated to E.coli beta-glucuronidase.
CloneAGP3
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone AGP3 is a mouse monoclonal antibody with specificity for polyehtylene glycol backbone.
IsotypeIgMκ
Species Reactivity
  • All
Species Reactivity NoteAll.
Antibody TypeMonoclonal Antibody
Purification MethodPurified by ion-exchange chromatography.
Molecular WeightN/A
Product Usage Statements
Quality AssuranceEvaluated by ELISA with immobilized Polyethylene Glycol (10K-PEG, 3K-PEG, and 2K-mPEG).

ELISA Analysis: 20 µg/mL of Polyethylene Glycol (PEG) (10K-PEG, 3K-PEG, and 2K-mPEG) were detected by this antibody.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Global Trade Item Number
Catalogue Number GTIN
MABS1963-25UG 04054839607837