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CBL1337-I Anti-PECAM-1 Antibody, clone 390

This rat monoclonal Anti-PECAM-1 Antibody, clone 390, Cat. No. CBL1337-I is validated for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, and Neutralization applications.

Anti-PECAM-1 Antibody, clone 390 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: Pecam1, Pecam, Pecam-1 UniProt Number: Q08481
CBL1337-I
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MFC, IF, IP, NEUTRPurifiedMonoclonal Antibody
Description
Catalogue NumberCBL1337-I
ReplacesCBL1337
DescriptionAnti-PECAM-1 Antibody, clone 390
Alternate Names
  • Platelet endothelial cell adhesion molecule
  • CD31
  • PECAM-1
Background InformationPlatelet endothelial cell adhesion molecule (UniProt Q08481; also known as CD31, PECAM-1) is encoded by the Pecam1 (also known as Pecam, Pecam-1) gene (Gene ID 18613) in murine species. PECAM-1 (CD31) is a 130-kD cell adhesion molecule that is expressed at the junctions of all continuous endothelia, as well as most bone marrow-derived hematopoietic cells, including platelets, monocytes, granulocytes and some lymphocytes. The human PECAM1 and murine Pecam1 genes both produce multiple spliced isoforms with varying lengths in cytoplasmic domain, with the isoform Long being the major form expressed in human tissues and endothelial cells (ECs), and isoform delta 14-15 being the predominantly expressed murine form. PECAM-1 plays an import role in maintaining vascular integrity and function by mediating ECs interactions with pericytes and smooth muscle cells (SMCs). PECAM-1 is initially produced with a signal peptide (human a.a. 1-27, murine a.a. 1-17) sequence, the removal of which yields the mature protein with a large extracellular region (human a.a. 28-601, mouse a.a. 18-590) composed of six C2-type Ig-like domains, followed by a transmembrane seqment (human a.a. 602-620, mouse a.a. 591-609) and a cytoplasmic tail (human a.a. 621-738, mouse a.a. 610-727) that plays an essential role in PECAM-1-mediated cellular signaling.
References
Product Information
FormatPurified
PresentationPurified rat IgG2a in PBS without azide.
Quality LevelMQ100
Applications
ApplicationThis rat monoclonal Anti-PECAM-1 Antibody, clone 390, Cat. No. CBL1337-I is validated for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, and Neutralization applications.
Key Applications
  • Flow Cytometry
  • Immunofluorescence
  • Immunoprecipitation
  • Neutralizing
Application NotesFlow Cytometry Analysis: A representative lot detected the expression of wild-type, ∆12,15 and ∆14,15 murine PECAM-1 constructs on the surface of transfected mouse L-cells (Baldwin, H.S., et al. (1994). Development. 120(9):2539-2553).

Immunofluorescence Analysis: A representative lot localized PECAM-1 immunoreactivity by fluorescent immunohistochemistry staining of murine embryo cryosections (Baldwin, H.S., et al. (1994). Development. 120(9):2539-2553).

Immunoprecipitation Analysis: A representative lot immunoprecipitated the 120-130 kDa exogenously expressed PECAM-1 ∆12,15 construct from transfected murine L-cells (Baldwin, H.S., et al. (1994). Development. 120(9):2539-2553).

Neutralizing Analysis: A representative lot inhibited the aggregation of murine L-cell transfectants expressing wild-type and ∆12,15, but not ∆14,15, murine PECAM-1 constructs (Baldwin, H.S., et al. (1994). Development. 120(9):2539-2553).

Clone 390 is also available as FITC conjugate (Cat. No. CBL1337F) for direct immunofluorescent staining in flow cytometry, immunocytochemistry and immunohistochemistry applications.
Biological Information
ImmunogenMouse leukocyte cell line 32D.
Epitopeextracellular domain
Clone390
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone 390 immunoprecipitated the characteristic 120-130 kDa protein from L-cells transfected with muPECAM-1∆12,15 and selectively stained murine vessels in acetone-fixed tissue sections (Baldwin, H.S., et al. (1994). Development. 120(9):2539-2553).
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Pecam1
  • Pecam
  • Pecam-1
Purification MethodProtein G purified.
UniProt Number
Molecular Weight79.49/76.14/78.23/68.05 kDa (mature isoform 1/2/3/4), 81.26/77.92/80.00/69.82 kDa (isoform 1/2/3/4 pro-form) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis: 2 µL of this antibody detected PECAM-1-positive monocytes among one million mouse splenocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CBL1337-I 04054839073526