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MABS2300-100UG Anti-Neuropilin-1 Antibody, ECD b1b2 Antibody, clone mAb2

Anti-Neuropilin-1, ECD b1b2, clone mAb2, Cat. No. MABS2301, is a mouse monoclonal antibody that detects Neuropilin-1 (NRP1) and is tested for use in ELISA, Immunocytochemistry, and Immunofluorescence.

MABS2300-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABS2300-100UG
DescriptionAnti-Neuropilin-1 Antibody, ECD b1b2 Antibody, clone mAb2
Alternate Names
  • Vascular endothelial cell growth factor 165 receptor
  • CD304
Background InformationNeuropilin-1 (UniProt: O14786; also known as Vascular endothelial cell growth factor 165 receptor, CD304) is encoded by the NRP1 (also known as NRP, VEGF165R) gene (Gene ID: 8829) in human. Neuropilins are highly conserved, non-tyrosine kinase, single-transmembrane glycoproteins that serve as co-receptors for various molecules, including semaphorins and vascular endothelial growth factors (VEGF). Neuropilin-1 is a single-pass type I, homodimeric, membrane glycoprotein that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form that contains a long extracellular domain (aa 22-856), a transmembrane domain (aa 857-879), and a cytoplasmic domain (aa 880-923). It acts as a receptor involved in the development of the cardiovascular system, angiogenesis, and formation of certain neuronal circuits. It is also reported to mediate the chemorepulsant activity of semaphorins. Neuropilin-1 has four well conserved domains: CUB (a1/a2; aa 27-141 and 147-265) domain, F5/8 (b1/b2; aa 275-424 and 431-583) domain, MAM domain (aa 645-811), and another domain that contains a transmembrane and a short cytoplasmic region. CUB domains mediate binding to semaphorins, while the tandem F5/8 domains are responsible for VEGF and heparin binding and recognizes a C-end role (CendR) motif R/KXXR/K on its ligands. The presence of the CUB (a1a2) domain is reported to enhance VEGF165 binding to F5/8 (b1b2) domain. The MAM domain is reported to mediate semaphorin signaling to Plexin-A. Neuropilin-1 has been recognized as an additional cellular mediator that facilitates the entry of SARS-CoV-2 into host cells. It binds to furin-cleaved spike protein and significantly potentiates SARS-CoV-2 infectivity. This effect can be blocked by monoclonal antibodies against the extracellular F5/8 (b1/b2) domain of Neuropilin-1 or with selective Neuropilin antagonists that bind to CendR binding b1 pocket. (Ref.: Kyrou, I., et al. (2021). Signal Trans. Targeted Ther. 6; Article number: 21; Daly, JL., et al. (2020). Science. 370(6518); 861-865; Cantuti-Castelvetri, L., et al. (2020). Science. 370(6518); 856-860; Mamluk, R., et al. (2002). J. Biol. Chem. 277(27); 24818-24825).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Neuropilin-1, ECD b1b2, clone mAb2, Cat. No. MABS2301, is a mouse monoclonal antibody that detects Neuropilin-1 (NRP1) and is tested for use in ELISA, Immunocytochemistry, and Immunofluorescence.
Key Applications
  • Immunocytochemistry
  • ELISA
  • Immunofluorescence
Application NotesTested Applications

ELISA Analysis: A representative lot detected Neuropilin-1 in ELISA applications (Daly, J.L., et al. (2020). Science. 370(6518):861-865).

Immunofluorescence Analysis: A representative lot detected Neuropilin-1 in Immunofluorescence applications (Cantuti-Castelvetri, L., et al. (2020). Science. 370(6518):856-860; Daly, J.L., et al. (2020). Science. 370(6518):861-865).

Immunocytochemistry Analysis: A representative lot detected Neuropilin-1 in Immunocytochemistry applications (Daly, J.L., et al. (2020). Science. 370(6518):861-865).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenHis-tagged recombinant fragment corresponding to 311 amino acids from the b1b2 region within the extracellular domain of human Neuropilin-1.
EpitopeExtracellular domain
ClonemAb2 (alternatively 3E7)
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone mAb2 is a mouse monoclonal antibody that detects Neuropilin-1. It targets an epitope within the b1b2 domain in the extracellular region.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NRP1
Purification MethodProtein G purified
UniProt Number
Molecular Weight103.13 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in PC-3 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Neuropilin-1 in PC-3 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS2300-100UG 04065267613925

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