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MABF3034-25UG Anti-NKG2D/CD314 Antibody, clone A10

Anti-NKG2D/CD314, clone A10, Cat. No. MABF3034, is an Armenian Hamster monoclonal antibody that detects NKG2D/CD314 and is tested for use in Flow Cytometry and Agonist Function.

MABF3034-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF3034-25UG
DescriptionAnti-NKG2D/CD314 Antibody, clone A10
Alternate Names
  • Tumor necrosis factor ligand superfamily member 13B
  • B-cell-activating factor
  • BAFF
  • CD257
Background InformationTumor necrosis factor ligand superfamily member 13B (UniProt: Q9WU72; also known as B-cell-activating factor, BAFF, CD257) is encoded by the Tnfsf13b (also known as Baff) gene (Gene ID: 24099) in murine species. BAFF is a single-pass type II, homotrimeric, membrane glycoprotein with a cytoplasmic domain (aa 1-47), a transmembrane domain (aa 48-68), and an extracellular domain (aa 69-309). A soluble form of BAFF has also been described that is produced by the cleavage of membrane form between amino acids 126-127. It is expressed by cells of myeloid origin and by stromal cells. Two isoforms of BAFF have been described that are produced by alternative splicing. Isoform 2 is reported to inhibit the secretion and bioactivity of isoform 1. BAFF is reported to bind to TNFRSF13B/TACI and TNFRSF17/BCMA. It serves as a potent B cell survival factor and is a critical immune checkpoint during peripheral B cell maturation. Its levels are often elevated in autoimmune diseases, such as rheumatoid arthritis and systemic lupus erythematosus (SLE). Elevated levels of BAFF are also observed in subjects with non-Hodgkin s lymphoma (NHL) and it is reported to promote survival of both NHL-derived B lymphoma cells and B cell chronic lymphocytic leukemia (B-CLL) cells. (Ref.: Vigolo, M., et al. (2018). Nat. Commun. 9(1); 1199; Batten, M., et al. (2004). J. Immunol. 172(2); 812-822; Gavin, AL., et al. (2003). J. Biol. Chem. 278(40); 38220-38228).
References
Product Information
FormatPurified
PresentationPurified Armenian Hamster monoclonal antibody IgG in PBS without azide.
Quality LevelMQ200
Applications
ApplicationAnti-NKG2D/CD314, clone A10, Cat. No. MABF3034, is an Armenian Hamster monoclonal antibody that detects NKG2D/CD314 and is tested for use in Flow Cytometry and Agonist Function.
Key Applications
  • Flow Cytometry
  • Inhibition
  • Agonist or Inhibitor
Application NotesTested applications

Flow Cytometry Analysis: A representative lot detected NKG2D/CD314 in Flow Cytometry applications (Ho, E.L., et al. (2002). J Immunol. 169(7):3667-75).

Agonist Function: A representative lot stimulated the production of GM-CSF by KY-1 NK clones and C57BL/6 LAK cells and also stimulated NK cells to kill Daudi targets. (Ho, E.L., et al. (2002). J Immunol. 169(7):3667-75).

Agonist Functon: A representative lot increased Vav phosphorylation in freshly isolated splenic C57BL/6 NK cells. (Ghasemi, R., et al. (2016). Nat Commun. 7:12878).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenRecombinant fragment containing the extracellular domain of mouse NKG2D/CD314.
EpitopeExtracellular domain
CloneA10
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostArmenian Hamster
SpecificityClone A10 is an Armenian Hamster monoclonal antibody that detects NKG2-D type II integral membrane protein (NKG2D/CD314).
IsotypeIgGκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Tnfsf13b
Purification MethodProtein G purified
UniProt Number
Molecular Weight26.71 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in C57BL/6 Mouse splenocytes.

Flow Cytometry Analysis (FC): 1.0 µg of this antibody detected NKG2D/CD314 in one million C57BL/6 mouse splenocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF3034-25UG 04065269125907