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ABE577 Sigma-AldrichAnti-Myb Binding Protein 1A Antibody

Anti-Myb Binding Protein 1A Antibody is a highly specific rabbit polyclonal antibody, that targets Myb-binding protein 1A & has been tested in western blotting & ICC.

Anti-Myb Binding Protein 1A Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References

Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: MYBBP1A, P160 UniProt Number: Q9BQG0

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, ICC, ChIP	Rb	Serum	Polyclonal Antibody

Description
Catalogue Number	ABE577
Description	Anti-Myb Binding Protein 1A Antibody
Alternate Names	Myb-binding protein 1A
Background Information	Myb-binding protein 1A (MYBBP1A) interacts with sequence specific DNA-binding proteins in order to activate or repress transcription. Transcriptional repression via Myb-binding protein 1A is thought to be mediated by histone deacetylase activity (HDAC activity).

References

Product Information
Format	Serum
Control	A431 cell lysate
Presentation	Rabbit polyclonal serum with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-Myb Binding Protein 1A Antibody is a highly specific rabbit polyclonal antibody, that targets Myb-binding protein 1A & has been tested in western blotting & ICC.
Key Applications	Western Blotting Immunocytochemistry Chromatin Immunoprecipitation (ChIP)
Application Notes	Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Myb Binding Protein 1A in diploid human lung embryonic fibroblasts (Prof. Atilla Nemeth, University of Regensburg, Germany.). Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Myb Binding Protein 1A in HeLa cells (Hochstatter, J., et al. (2012). J Biol Chem. 287(29):24365-24377.). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot from an independent laboratory immunoprecipitated Myb Binding Protein 1A in ChIP (Hochstatter, J., et al. (2012). J Biol Chem. 287(29):24365-24377.).

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to human Myb Binding Protein 1A.
Host	Rabbit
Specificity	This antibody detects the full length and cleaved forms of Myb Binding Protein 1A.
Species Reactivity	Human
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_001099008
Gene Symbol	MYBBP1A P160
Purification Method	Unpurified
UniProt Number	Q9BQG0
Molecular Weight	~160 kDa observed. Uncharacterized band(s) may be observed in some cell lysates. The full length of this protein is observed at ~160 kDa; however, it is proteolytically cleaved into ~140 kDa and ~67 kDa fragments (Hochstatter, J., et al. (2012). J Biol Chem. 287(29):24365-24377.; Mori, S., et al. (2012). PLoS One. 7(10):e39723.).

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in A431 cell lysate. Western Blotting Analyis: A 1:1,000 dilution from a representative lot detected Myb Binding Protein 1A in 10 µg of A431 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
ABE577	04053252913952

Documentation

Anti-Myb Binding Protein 1A Antibody SDS

Title
Safety Data Sheet (SDS)

Anti-Myb Binding Protein 1A Antibody Certificates of Analysis

Title	Lot Number
Anti-Myb Binding Protein 1A - Q2204800	Q2204800
Anti-Myb Binding Protein 1A Polyclonal Antibody	3049350

References

Reference overview	Pub Med ID
Myb-binding protein 1a (Mybbp1a) regulates levels and processing of pre-ribosomal RNA. Hochstatter, Julia, et al. J. Biol. Chem., 287: 24365-77 (2012) 2012 Show Abstract Ribosomal RNA gene transcription, co-transcriptional processing, and ribosome biogenesis are highly coordinated processes that are tightly regulated during cell growth. In this study we discovered that Mybbp1a is associated with both the RNA polymerase I complex and the ribosome biogenesis machinery. Using a reporter assay that uncouples transcription and RNA processing, we show that Mybbp1a represses rRNA gene transcription. In addition, overexpression of the protein reduces RNA polymerase I loading on endogenous rRNA genes as revealed by chromatin immunoprecipitation experiments. Accordingly, depletion of Mybbp1a results in an accumulation of the rRNA precursor in vivo but surprisingly also causes growth arrest of the cells. This effect can be explained by the observation that the modulation of Mybbp1a protein levels results in defects in pre-rRNA processing within the cell. Therefore, the protein may play a dual role in the rRNA metabolism, potentially linking and coordinating ribosomal DNA transcription and pre-rRNA processing to allow for the efficient synthesis of ribosomes.	22645127

Reference overview

Pub Med ID

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