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MAB3731 Anti-Met/HGFR Antibody, phospho-specific (Tyr1234/1235), clone 6AT1877

Anti-Met/HGFR Antibody, phospho-specific (Tyr1234/1235), clone 6AT1877 detects level of Met/HGFR & has been published & validated for use in ELISA.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Growth Factors & Receptors Gene Symbol: MET, AUTS9, c-Met, RCCP2, HGFR, EC 2.7.10.1 UniProt Number: P08581
MAB3731
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MELISAMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB3731
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Met/HGFR Antibody, phospho-specific (Tyr1234/1235), clone 6AT1877
Alternate Names
  • c-Met
  • cmet
  • HGFR
  • RCCP2
  • met proto-oncogene (hepatocyte growth factor receptor)
References
Product Information
FormatPurified
HS Code3002 15 90
PresentationProtein G purified immunoglobulin. Liquid in PBS containing 0.09% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Met/HGFR Antibody, phospho-specific (Tyr1234/1235), clone 6AT1877 detects level of Met/HGFR & has been published & validated for use in ELISA.
Key Applications
  • ELISA
Application NotesELISA (direct): 1:1,000

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenSynthetic phospho-peptide corresponding to residues surrounding Tyr1234/1235 of human Met.
Epitopephospho-specific (Tyr1234/1235)
Clone6AT1877
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts with Met/HGFR (Hepatocyte growth factor receptor) only when phosphorylated at Tyr1234 and Tyr1235. The Met receptor tyrosine kinase is the prototypical member of a small subfamily of growth factor receptors that when activated, induces mitogenic, motogenic, and morphogenic cellular responses. The ligand for Met is hepatocyte growth factor/scatter factor (HGF/SF) and while normal HGF/SF-Met signaling is required for embryonic development, abnormal Met signaling has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes.
IsotypeIgG1
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma.
Gene Symbol
  • MET
  • AUTS9
  • c-Met
  • RCCP2
  • HGFR
  • EC 2.7.10.1
Modifications
  • Phosphorylation
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P08581 # Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity.
SIZE: 1390 amino acids; 155527 Da
SUBUNIT: Heterodimer formed of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1 and GRB2. Interacts with SPSB1, SPSB2 and SPSB4 (By similarity). Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1356, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DISEASE: SwissProt: P08581 # Activation of MET after rearrangement with the TPR gene produces an oncogenic protein. & Defects in MET may be associated with gastric cancer. & Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550]. & Defects in MET are a cause of hereditary papillary renal carcinoma (HPRC) [MIM:605074]; also known as papillary renal cell carcinoma 2 (RCCP2). HPRC is a form of inherited kidney cancer characterized by a predisposition to develop multiple, bilateral papillary renal tumors. The pattern of inheritance is consistent with autosomal dominant transmission with reduced penetrance. & Genetic variations in MET may be associated with susceptibility to autism type 1B (AUTS1B) [MIM:608636]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior.
SIMILARITY: SwissProt: P08581 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. & Contains 3 IPT/TIG domains. & Contains 1 protein kinase domain. & Contains 1 Sema domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB3731 04053252617270

Documentation

Anti-Met/HGFR Antibody, phospho-specific (Tyr1234/1235), clone 6AT1877 SDS

Title

Safety Data Sheet (SDS) 

Anti-Met/HGFR Antibody, phospho-specific (Tyr1234/1235), clone 6AT1877 Certificates of Analysis

TitleLot Number
MOUSE ANTI-PHOSPHO-MET/HGFR (TYR1234/1235) MONOCLONAL ANTIBODY - 2395639 2395639
MOUSE ANTI-PHOSPHO-MET/HGFR (TYR1234/1235) - 3732971 3732971

References

Reference overviewPub Med ID
EGF and bFGF pre-treatment enhances neural specification and the response to neuronal commitment of MIAMI cells.
Gaëtan J-R Delcroix,Kevin M Curtis,Paul C Schiller,Claudia N Montero-Menei
Differentiation; research in biological diversity  80  2001

Show Abstract 		20813449 20813449

User Guides

Title
MOUSE ANTI-PHOSPHO-MET/HGFR (TYR1234/1235) MONOCLONAL ANTIBODY