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ABE333 Anti-MeCP2 Antibody, Isoform B

Detect MeCP2 using this rabbit polyclonal antibody, Anti-MeCP2 Antibody & Isoform B validated for use in western blotting.

Anti-MeCP2 Antibody, Isoform B MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: MECP2 UniProt Number: P51608-2
ABE333
200 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE333
DescriptionAnti-MeCP2 Antibody, Isoform B
Alternate Names
  • Methyl-CpG-binding protein 2
  • MeCp-2 protein
  • MeCp2
Background InformationDNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA and repress transcription both directly and by association with known corepressor proteins which include members of the histone deacetylase protein families.
References
Product Information
FormatAffinity Purified
Control
  • MCF7 cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect MeCP2 using this rabbit polyclonal antibody, Anti-MeCP2 Antibody & Isoform B validated for use in western blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human MeCP2.
EpitopeN-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes isoform B of MeCP2, but does not recognize isoform A. Additionally, this antibody recognizes the N-terminus of isoform B.
Species Reactivity
  • Human
Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse and Rabbit based on 100% sequence homology. Other homologies: Rat (94% sequence homology).
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MECP2
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~75 kDa observed. The calculated molecular weight is 53 kDa, however MeCP2 has been shown as a ~75 kDa band in western blots (Jarrar, M. H., et al. (2003). J Child Neurol. 18(10):675-682)(Zachariah, R., et al. (2012). PLOS. 7(11):1-10).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected MeCP2 in 10 µg of MCF7 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE333 04053252862922