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MAB4334Z Anti-MDR1 Antibody, clone UIC2, azide free

Detect MDR1 using this Anti-MDR1 Antibody, clone UIC2, azide free validated for use in FC, IF, IP, IH.

Anti-MDR1 Antibody, clone UIC2, azide free MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Metabolism Research Sub-Category: Toxicology & Drug Resistance Gene Symbol: ABCB1, PGY1, MGC163296, CD243, GP170, P-gp, MDR1, CLCS, ABC20, EC 3.6.3.44 UniProt Number: P08183
MAB4334Z
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, PmFC, IF, IP, IHC, FUNCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB4334Z
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-MDR1 Antibody, clone UIC2, azide free
Alternate Names
  • P-glycoprotein
  • CD243
  • p170
  • Pgp
  • MAB4334
Background InformationUIC2 is a mouse monoclonal antibody directed against the extracellular conformational epitope of P-glycoprotein (Pgp). This antibody can be used in flow cytometry, immunofluorescence microscopy and immunohistochemistry in frozen and paraffin sections to detect Pgp expression on the membrane of MDR1 positive cells. In functional experiments, UIC2 was shown to inhibit Pgp-mediated efflux activity and potentiate the cytotoxic effects of chemotherapy drugs transported by Pgp. Additionally, the UIC2 monoclonal antibody can be used in combination with MDR1 substrates for simulatenaeous detection of Pgp expression and function by flow cytometry (MILLIPORE's UIC2 Shift assay - see product ECM905).
References
Product Information
FormatPurified
PresentationPurified immunoglobulin from Protein A Sepharose™ chromatography. Sterile filtered in 0.01 M PBS, no preservative.
Quality LevelMQ300
Applications
ApplicationDetect MDR1 using this Anti-MDR1 Antibody, clone UIC2, azide free validated for use in FC, IF, IP, IH.
Key Applications
  • Flow Cytometry
  • Immunofluorescence
  • Immunoprecipitation
  • Immunohistochemistry
  • Affects Function
Application NotesInhibition of P-glycoprotein efflux activity in functional experiments: 10 - 100 μg/mL, varies in different systems

Immunoprecipitation: 5 - 10 fold molar excess of UIC2 over the expected Pgp concentration; realistically, 1 - 5 ug per 1 μl of cell lysate

Flow Cytometry (conventional and UIC2 Shift assay): depends highly on Pgp expression by target cells; usually, 0.1 μg/mL for low Pgp expressors and 1 μg/mL for high Pgp expressors

Immunofluorescence: depends highly on Pgp expression by target cells; usually, 0.1 μg/mL for low Pgp expressors and 1 μg/mL for high Pgp expressors IHC on frozen and paraffin sections: 1 - 10 μg/mL
Biological Information
ImmunogenMouse BALB/c 3T3 fibroblasts transfected with human MDR1 cDNA, followed by a multistep selection of tranfectants for resistance to vinblastine.
Epitopeconformational extracellular epitope, azide free
CloneUIC2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityP-glycoprotein encoded by the MDR1 gene (ABCB1; LocusLink ID: 5243)
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Primate
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.
Gene Symbol
  • ABCB1
  • PGY1
  • MGC163296
  • CD243
  • GP170
  • P-gp
  • MDR1
  • CLCS
  • ABC20
  • EC 3.6.3.44
Non-Reactive Species
  • Mouse
  • Rat
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P08183 # Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
SIZE: 1280 amino acids; 141463 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in liver, kidney, small intestine and brain.
SIMILARITY: SwissProt: P08183 ## Belongs to the ABC transporter family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. & Contains 2 ABC transmembrane type-1 domains. & Contains 2 ABC transporter domains.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C for up 12 months.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB4334Z 04053252723605

Documentation

Anti-MDR1 Antibody, clone UIC2, azide free SDS

Title

Safety Data Sheet (SDS) 

Anti-MDR1 Antibody, clone UIC2, azide free Certificates of Analysis

TitleLot Number
Mouse Monoclonal Anti-UIC2 (MDR1 P-glycoprotein) SODIUM AZIDE FREE Mouse Monoclonal Antibody - 2379332 2379332
Mouse Monoclonal Anti-UIC2 (MDR1 P-glycoprotein) - 3725267 3725267
Mouse Monoclonal Anti-UIC2 (MDR1 P-glycoprotein) -2524062 2524062
Mouse Monoclonal Anti-UIC2 (MDR1 P-glycoprotein) -2624591 2624591
Mouse Monoclonal Anti-UIC2 (MDR1 P-glycoprotein) -2805164 2805164
Mouse Monoclonal Anti-UIC2 (MDR1 P-glycoprotein) -2810621 2810621
Mouse Monoclonal Anti-UIC2 (MDR1 P-glycoprotein) SODIUM AZIDE FREE - 3321109 3321109
Mouse Monoclonal Anti-UIC2 (MDR1 P-glycoprotein) SODIUM AZIDE FREE - 3702343 3702343
Mouse Monoclonal Anti-UIC2 (MDR1 P-glycoprotein) SODIUM AZIDE FREE Mouse Monoclonal Antibody 2879769
Mouse Monoclonal Anti-UIC2 (MDR1 P-glycoprotein) SODIUM AZIDE FREE Mouse Monoclonal Antibody 2891001
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MAB4334 Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2

Alternative Format

Catalogue Number Description
MAB4334 Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies