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MABT1550-25UL Anti-Lactadherin/MFGE8 Antibody, clone 5C5

Anti-Lactadherin/MFGE8 , clone 5C5, Cat. No. MABT1550, is a mouse monoclonal antibody that detects Lactadherin and is tested for use in Immunofluorescence and Western Blotting.

MABT1550-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABT1550-25UL
DescriptionAnti-Lactadherin/MFGE8 Antibody, clone 5C5
Alternate Names
  • Lactadherin
  • MFGM
  • Milk fat globule-EGF factor 8
  • MFG-E8
  • SED1
  • Sperm surface protein SP47
  • PP47
Background InformationLactadherin (UniProt: P79385; also known as MFGM, Milk fat globule-EGF factor 8, MFG-E8, SED1, Sperm surface protein SP47, PP47) is encoded by the MFGE8 gene (Gene ID: 397545) in Boar. MFG-E8 is a peripheral membrane glycoprotein that is widely expressed in many cell types. At its N-terminal region, it contains two EGF-like repeats (aa 2-41 and 44-88). The second EGF repeat contains an RGF integrin-binding domain that allows its interaction with and activation of Vβ3 and Vβ5 integrins. It also has two C-terminal F5/8 type C domains. The second F5/8 type C domain exhibits high affinity for binding to phosphatidylserine present in cell membranes. MFG-E8 displays high affinity for glycoproteins on egg surface and contributes to the ability of sperm to adhere to the egg in an integrin-independent manner. It has been reported that although MFG-E8 is not absolutely essential for fertilization, its absence is shown to reduce fertility in mice. It has also been implicated in a variety of other physiological functions. MFG-E8 can bind apoptotic cells via its F5/8C domains, and its RGD motif is shown to be essential for cell engulfment by macrophages. In the mammary glands, it is required for clearance of apoptotic cells during mammary gland involution at the end of lactation. Defects in or lack of MFG-E8 can cause the accumulation of apoptotic cell remnants, duct distension and mastitis. (Ref.: Zigo, M., et al. (2015). Cell Tissue Res. 359(3); 895-908; Ensslin, MA., and Shur, BD. (2007). Proc. Natl. Acad. Sci. USA 104(8); 2715-2720).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Lactadherin/MFGE8 , clone 5C5, Cat. No. MABT1550, is a mouse monoclonal antibody that detects Lactadherin and is tested for use in Immunofluorescence and Western Blotting.
Key Applications
  • Western Blotting
  • Immunofluorescence
Application NotesTested Applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected Lactadherin/MFGE8 in HeLa cell lysate.

Western Blotting Analysis: A representative lot detected Lactadherin/MFGE8 in Western Blotting applications (Zigo, M., et al. (2015). Cell Tissue Res. 359(3):895-908).

Immunofluorescence Analysis: A representative lot detected Lactadherin/MFGE8 in Immunofluorescence applications (Zigo, M., et al. (2015). Cell Tissue Res. 359(3):895-908).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenPurified Sperm surface protein from Boar.
EpitopeUnknown
Clone5C5
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 5C5 is a mouse monoclonal antibody that detects Sperm surface protein (Lactadherin).
IsotypeIgG1κ
Species Reactivity
  • Human
  • Porcine
Species Reactivity NoteHuman, Boar.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • MFGE8
Purification MethodProtein G purified
UniProt Number
Molecular Weight~47 kDa observed; 45.73 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in MDA-MB-231 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Lactadherin/MFGE8 in MDA-MB-231 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT1550-25UL 04065267327822