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MABF242 Anti-LST1, clone 7E2 Antibody

MABF242
100 µg  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF242
DescriptionAnti-LST1, clone 7E2 Antibody
Alternate Names
  • Leukocyte-specific transcript 1 protein
  • Protein B144
Background InformationLST1, also known as Leukocyte-specific transcript 1 protein, Protein B144, and encoded by the gene LST1/B144, is an interesting protein expressed in many tissues in both the developing fetus as well as the adult. Over-expression induces morphological changes in many cells types that include the production of filopodia and microspikes. LST1 is also involved in dendritic cell maturation and also has anti-proliferation activity on lymphocyte proliferation. LST1 is localized to internal and Golgi membranes and is primarily a single-pass membrane bound protein. Recent research also demonstrates that LST1 is important for the assembly of tunneling nanotubes, a recently discovered new mechanism of cell-cell communication. LST1 induces nanotube formation by recruiting the small GTPase RalA to the plasma membrane and promoting its interaction with the exocyst complex. Furthermore, LST1 recruits the actin-crosslinking protein filamin to the plasma membrane and interacts with M-Sec, myosin and myoferlin, and this suggests a molecular model for nanotube generation and LST1’s essential role in their formation and maintenance.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-LST1 antibody, clone 7E2 detects level of LST1 & has been published & validated for use in LST1.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected LST1 in 10 µg of human brain, human lung, human liver, human kidney, human skin, human spleen, human thymus, human pancreas, human ovary, human testis, human small intestine, human parathyroid, and human adrenal gland tissue lysate.
Western Blotting Analysis: A representative lot detected LST1 in HeLa cell lysate (Schiller, C., et al. (2009). Hybridoma. 28(4):281-286).
Biological Information
ImmunogenHis-tagged recombinant protein corresponding to human LST1.
Clone7E2
Concentration1 mg/mL
HostRat
IsotypeIgG2bκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • LST1
  • B144
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~26 kDa observed. The target is universal expressed, not leukocytes specific LST1 forms dimers through disulfide bridges.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human heart tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected LST1 in 10 µg of human heart tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF242 04053252976346

Documentation

Anti-LST1, clone 7E2 Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-LST1, clone 7E2 Antibody Certificates of Analysis

TitleLot Number
Anti-LST1, clone 7E2 - Q2430676 Q2430676

References

Reference overviewPub Med ID
LST1 promotes the assembly of a molecular machinery responsible for tunneling nanotube formation.
Schiller, C; Diakopoulos, KN; Rohwedder, I; Kremmer, E; von Toerne, C; Ueffing, M; Weidle, UH; Ohno, H; Weiss, EH
Journal of cell science  126  767-77  2013

Show Abstract
23239025 23239025
Rat monoclonal antibodies specific for LST1 proteins.
Schiller, C; Nitschké, MJ; Seidl, A; Kremmer, E; Weiss, EH
Hybridoma (2005)  28  281-6  2009

Show Abstract
19663701 19663701