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MABS2066-25UG Anti-LLGL1 Antibody, clone mAb17-35

Anti-LLGL1, clone mAb17-35, Cat. No. MABS2066, is a mouse monoclonal antibody that detects Lethal(2) giant larvae protein homolog 1 and has been tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Anti-LLGL1 Antibody, clone mAb17-35 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: Llgl1, Llglh UniProt Number: Q80Y17
MABS2066-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MICC, IHC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS2066-25UG
DescriptionAnti-LLGL1 Antibody, clone mAb17-35
Alternate Names
  • Lethal(2) giant larvae protein homolog 1
  • Mgl-1
  • Mlgl
Background InformationLethal(2) giant larvae protein homolog 1 (UniProt: Q80Y17; also known as LLGL, Mgl-1, Mlgl) is encoded by the Llgl1 (also known as Llglh) gene (Gene ID: 16897) in murine species. LLGL belongs to the WD repeat L(2)GL family. LLGL is a cortical cytoskeleton protein that is found in a complex involved in maintenance of cell polarity and epithelial integrity. It is involved in the regulation of mitotic spindle orientation, proliferation, differentiation, and tissue organization of neuroepithelial cells. It is generally enriched in developing axons. LLGL stability and function is achieve by its interaction with RanBPM protein, which primarily acts as a scaffolding protein. Its N-terminal region containing SPRY domain is shown to be responsible for binding to RanBPM and this binding functionally extends its half-life by preventing its turnover through ubiquitin-proteasomal pathway. RanBPM is shown enhance tumor suppressor activity of LLGL by inhibiting LLGL-mediated cell proliferation and cell migration. (Ref.: Suresh, B., et al. (2010). J. Biol. Chem. 285(46); 35340-35349).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-LLGL1, clone mAb17-35, Cat. No. MABS2066, is a mouse monoclonal antibody that detects Lethal(2) giant larvae protein homolog 1 and has been tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected LLGL1 in Western Blotting applications (Ivanov, A.I., et. al. (2010). Am J Pathol. 176(1):134-45).

Immunocytochemistry Analysis: A representative lot detected LLGL1 in Immunocytochemistry applications (Ivanov, A.I., et. al. (2010). Am J Pathol. 176(1):134-45).

Immunohistochemistry Analysis: A representative lot detected LLGL1 in Immunohistochemistry applications (Weimer, J.M., et. al. (2009). Development. 136(17):2965-75).
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 153 amino acids from the C-terminal region of murine Lethal(2) giant larvae protein homolog 1 (LLGL).
EpitopeC-terminus
ClonemAb17-35
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone mAb17-35 specifically detects LLGL1 in mouse. It targets an epitope with in 153 amino acids from the C-terminal region.
IsotypeIgG2bκ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Llgl1
  • Llglh
Purification MethodProtein G purified
UniProt Number
Molecular Weight~120 kDa observed; 112.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected LLGL1 in mouse brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS2066-25UG 04054839563256