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MABF2207-25UL Anti-LAG-3 Antibody, clone 1B5

Anti-LAG-3, clone 1B5, Cat. No. MABF2207, is a mouse monoclonal antibody that detects Lymphocyte activation gene 3 protein (LAG-3) and is tested for use in Flow Cytometry, Immunohistochemistry, Inhibition Assay, and Western Blotting.

MABF2207-25UL
25 µL  
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Overview

Replacement Information
Description
Catalogue NumberMABF2207-25UL
DescriptionAnti-LAG-3 Antibody, clone 1B5
Alternate Names
  • Lymphocyte activation gene 3 protein
  • FDC
  • CD223
Background InformationLymphocyte activation gene 3 protein (UniProt: P18627; also known as LAG-3, CD223) is encoded by the LAG3 (also known as FDC) gene (Gene ID: 3902) in human. LAG3 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 23-450), a transmembrane domain (aa 451-471), and a cytoplasmic domain (aa 472-525). It contains three Ig-like C2-type domains and one Ig-like V-type domain. LAG3 is primarily expressed in activated T-cells and in a subset of natural killer cells. Its expression is induced by IL-2, IL-7, and IL-12 on activated T-cells. Almost all CD4+ cells expressing LAG3 also express CD25. However, only about 10% of CD4+ cells lacking LAG3 express CD25. LAG3 serves as an inhibitory receptor on antigen activated T-cells and delivers inhibitory signals upon binding to ligands. Fibrinogen-like protein 1 (FGL1) is shown to be a major ligand for LAG3. LAG3 is reported to suppresses homeostasis proliferation (HP) of CD4 and CD8 lymphocytes and some dendritic cell populations. Mice deficient in LAG3 display enhanced HP. Following TCR engagement, it is shown to associate with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. LAG3 can be proteolytically cleaved by ADAM10 and ADAM17 within the connecting peptide region to generate a secretory form (sLAG3). ADAM10 is reported to mediate constitutive cleavage, but cleavage increases following T-cell activation. On the other hand, cleavage by ADAM17 is induced by TCR signaling in a Protein Kinase C theta (PRKCQ)-dependent manner. (Ref.: Scurr, M., et al. (2014). Mucosal Immunol. 7(2); 428-439; Goldberg, MV., and Drake, CG (2011). Curr. Top. Microbiol. Immunol. 344; 269-278).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in PBS without azide.
Quality LevelMQ200
Applications
ApplicationAnti-LAG-3, clone 1B5, Cat. No. MABF2207, is a mouse monoclonal antibody that detects Lymphocyte activation gene 3 protein (LAG-3) and is tested for use in Flow Cytometry, Immunohistochemistry, Inhibition Assay, and Western Blotting.
Key Applications
  • Flow Cytometry
  • Immunohistochemistry
  • Western Blotting
  • Inhibition
Application NotesFlow Cytometry Analysis: 1 µg/mL from a representative lot detected LAG-3 in JRT cells transduced with LAG-3 (Courtesy of Cardiff University, United Kingdom).

Inhibition Analysis: SPR binding of LAG-3:Fc to immobilised MHC-II molecules. Blockade of LAG-3:Fc interaction with HLA-DR1 by binding of titrating anti-LAG-3 antibody with LAG-3:Fc analyte before injection over HLA-DR1 coated CM5 sensor chip (Courtesy of Cardiff University, United Kingdom).

Immunohistochemistry Applications: A representative lot detected LAG-3 in Human colon and human colorectal cancer (Courtesy of Cardiff University, United Kingdom).

Western Blotting Analysis: 2 µg/mL from a representative lot detected LAG-3 in JRT cells transduced with LAG-3 (Courtesy of Cardiff University, United Kingdom).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenFull-length human recombinant Lymphocyte activation gene 3 protein (LAG-3)-Fc fusion protein.
EpitopeExtracellular domain
Clone1B5
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 1B5 is a mouse monoclonal antibody that detects human Lymphocyte activation gene 3 protein (LAG-3). It targets an epitope within the extracellular domain.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • LAG3
  • FDC
Purification MethodProtein G purified
UniProt Number
Molecular Weight57.45 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis (FC): 1 µg of this antibody detected LAG-3 in one million human peripheral blood mononuclear cells (PBMC).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2207-25UL 04065265237697