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420400 Anti-KDEL Mouse mAb (10C3)

This Anti-KDEL Mouse mAb (10C3) is validated for use in Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of KDEL.

Anti-KDEL Mouse mAb (10C3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
420400
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Ca, Dr, Gp, Ht, H, Mk, M, Porcine, Rb, R, Sh, YeastMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
420400-50UG Plastic ampoule 50 μg
Description
OverviewRecognizes the Grp78 (BiP) and Grp94 proteins.
Catalogue Number420400
Brand Family Calbiochem®
Application Data
Detection of KDEL by immunoblotting. Samples: Recombinant Grp78 (BiP) protein (lane 1), whole cell lysate RK-13 cells (lane 2), mouse liver microsomes (lane 3), rat liver microsomes (lane 4), and whole cell lysate from heat shocked HeLa cells (lane 5). Primary antibody: Anti-KDEL Mouse mAb (10C3) (Cat. No. 420400) (1:1000). Detection: chemiluminescence.
References
ReferencesKuznetsov, G., et al. 1994. J. Biol. Chem. 269, 22990.
Huovila, A.-P., et al. 1992. J. Cell Biol. 118, 1305.
Munro, S., and Pelham, H.R.B. 1987. Cell 48, 899.
Munro, S., and Pelham, H.R.B. 1986. Cell 46, 291.
Product Information
FormLiquid
FormulationIn PBS, 50% glycerol.
Positive controlRK-13 cells, Mouse liver microsomes, Rat liver microsomes, Heat shocked HeLa cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Application NotesImmunoblotting (4 µg/ml, colorimetric)
Immunocytochemistry (5 µg/ml)
Immunoprecipitation (5 µg/ml)
Application CommentsAlso recognizes unidentified proteins at ~55 kDa and ~43 kDa by immunoblotting. Does not cross-react with Hsp70. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic peptide (SEKDEL) corresponding to amino acids 649-654 of rat Grp78, conjugated to KLH
ImmunogenRat
Clone10C3
HostMouse
IsotypeIgG2a
Species Reactivity
  • Canine
  • Drosophila
  • Guinea Pig
  • Hamster
  • Human
  • Monkey
  • Mouse
  • Porcine
  • Rabbit
  • Rat
  • Sheep
  • Yeast
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Highly Toxic
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
420400-50UG 04055977187571

Documentation

Anti-KDEL Mouse mAb (10C3) Certificates of Analysis

TitleLot Number
420400

References

Reference overview
Kuznetsov, G., et al. 1994. J. Biol. Chem. 269, 22990.
Huovila, A.-P., et al. 1992. J. Cell Biol. 118, 1305.
Munro, S., and Pelham, H.R.B. 1987. Cell 48, 899.
Munro, S., and Pelham, H.R.B. 1986. Cell 46, 291.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-August-2007 RFH
ApplicationImmunoblotting (4 µg/ml, colorimetric)
Immunocytochemistry (5 µg/ml)
Immunoprecipitation (5 µg/ml)
Application Data
Detection of KDEL by immunoblotting. Samples: Recombinant Grp78 (BiP) protein (lane 1), whole cell lysate RK-13 cells (lane 2), mouse liver microsomes (lane 3), rat liver microsomes (lane 4), and whole cell lysate from heat shocked HeLa cells (lane 5). Primary antibody: Anti-KDEL Mouse mAb (10C3) (Cat. No. 420400) (1:1000). Detection: chemiluminescence.
DescriptionProtein G purified mouse monoclonal antibody. Recognizes the KDEL Grp78 (BiP) and Grp94 proteins.
BackgroundSeveral resident proteins in the ER are involved in the proper assembly and folding of newly synthesized proteins. These include the glucose-regulated protein 94 (Grp94), BiP, which functions in protein oligomerization, and protein disulfide isomerase (PDI), which catalyzes the rearrangement of the disulfide bond. Grp94, BiP, and PDI share a carboxy-terminal sequence (Lys-Asp-Glu-Leu) with other resident ER proteins. The KDEL sequence is sufficient for the retention of a protein in the ER.
HostMouse
Immunogen speciesRat
Immunogena synthetic peptide (SEKDEL) corresponding to amino acids 649-654 of rat Grp78, conjugated to KLH
Clone10C3
IsotypeIgG2a
SpeciesDrosophila, canine, guinea pig, hamster, human, monkey, mouse, porcine, rabbit, rat, sheep, yeast
Positive controlRK-13 cells, Mouse liver microsomes, Rat liver microsomes, Heat shocked HeLa cells
FormLiquid
FormulationIn PBS, 50% glycerol.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAlso recognizes unidentified proteins at ~55 kDa and ~43 kDa by immunoblotting. Does not cross-react with Hsp70. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Highly Toxic
ReferencesKuznetsov, G., et al. 1994. J. Biol. Chem. 269, 22990.
Huovila, A.-P., et al. 1992. J. Cell Biol. 118, 1305.
Munro, S., and Pelham, H.R.B. 1987. Cell 48, 899.
Munro, S., and Pelham, H.R.B. 1986. Cell 46, 291.