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MAB2056Z Anti-Integrin α3 Antibody, clone ASC-1 (Azide Free)

Anti-Integrin α3 Antibody, clone ASC-1 (Azide Free) inhibits cell attachment & detects level of Integrin α3 & has been published & validated for use in FC, IP, IH, Inhibition Assays.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Integrins Gene Symbol: ITGA3, GAP-B3, CD49c, FLJ34704, MSK18, VLA3a, VL3A, VCA-2, FLJ34631, FRP-2, GAPB3, CD49C UniProt Number: P26006
MAB2056Z
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, IP, IHC, FUNCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB2056Z
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Integrin α3 Antibody, clone ASC-1 (Azide Free)
Alternate Names
  • CD49c
Product Information
FormatPurified
PresentationPurified cultured supernatant in PBS without preservatives
Quality LevelMQ100
Applications
ApplicationAnti-Integrin α3 Antibody, clone ASC-1 (Azide Free) inhibits cell attachment & detects level of Integrin α3 & has been published & validated for use in FC, IP, IH, Inhibition Assays.
Key Applications
  • Flow Cytometry
  • Immunoprecipitation
  • Immunohistochemistry
  • Affects Function
Application NotesInhibition (Functional Activity): A previous lot of this antibody was shown to inhibit cell attachment by inhibiting SCC9 cell adhesion to human and EHS laminin, and laminin GD-2 peptide and potentiates the inhibition of SKOV-3 cell adhesion to EHS laminin by anti-integrin alpha 5 antibody GoH3. This antibody does not inhibit cell adhesion to fibronectin or collagen type IV.

Immunohistochemistry Analysis: A previous lot of this antibody was used on acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue.

Flow Cytometry Analysis: A previous lot of this antibody stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelial cells (HUVEC).

Immunoprecipitation Analysis: A previous lot of this antibody precipitates PAGE bands of 130 kDa (reduced) or 116 kDa and 135 kDa (non-reduced) from surface-biotinylated SCC9 or SKOV-3 cells.
Biological Information
ImmunogenHuman squamous cell carcinoma cells (SCC9)
CloneASC-1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityHuman alpha3 integrin (CD49c)
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryITGA3 encodes the integrin alpha 3 chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 3 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form an integrin that interacts with many extracellular-matrix proteins. The alpha 3 beta 1 integrin is known variously as: very late (activation) antigen 3 ('VLA-3'), very common antigen 2 ('VCA-2'), extracellular matrix receptor 1 ('ECMR1'), and galactoprotein b3 ('GAPB3').
Gene Symbol
  • ITGA3
  • GAP-B3
  • CD49c
  • FLJ34704
  • MSK18
  • VLA3a
  • VL3A
  • VCA-2
  • FLJ34631
  • FRP-2
  • GAPB3
  • CD49C
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P26006 # Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Alpha- 3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration.
SIZE: 1066 amino acids; 118698 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-3 associates with beta-1. Interacts with HPS5.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Isoform alpha-3A is widely expressed. Isoform alpha-3B is expressed in brain and heart. In brain, both isoforms are exclusively expressed on vascular smooth muscle cells, whereas in heart isoform alpha-3A is strongly expressed on vascular smooth muscle cells, isoform alpha-3B is detected only on endothelial vein cells.
PTM: Isoform alpha-3A, but not isoform alpha-3B, is phosphorylated on serine residues. Phosphorylation increases after phorbol 12- myristate 13-acetate stimulation.
SIMILARITY: SwissProt: P26006 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
Molecular Weight116 kDa
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20°C in undiluted aliquots.
Avoid repeated freeze-thaw cycles.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
MAB2056Z 04053252939600