Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABF2295-25UG Anti-IL-12 p40 Antibody, clone 11B10

Anti-IL-12 p40, clone 11B10, Cat. No. MABF229, is a mouse monoclonal antibody that detects Interleukin-12 subunit beta and has been tested for use in Flow Cytometry and Immunohistochemistry (Paraffin).

MABF2295-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2295-25UG
DescriptionAnti-IL-12 p40 Antibody, clone 11B10
Alternate Names
  • Interleukin-12 subunit beta
  • IL-12B
  • Cytotoxic lymphocyte maturation factor 40 kDa subunit
  • CLMF p40
  • IL-12 subunit p40
  • NK cell stimulatory factor chain 2
  • NKSF2
Background InformationInterleukin-12 subunit beta (UniProt: P29460; also known as IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, CLMF p40, IL-12 subunit p40, NK cell stimulatory factor chain 2, NKSF2) is encoded by the IL12B (also known as NKSF2) gene (Gene ID: 3593) in human. IL-12 is a heterodimeric protein composed of the p35 and p40 subunits. It is secreted by activated monocytes, macrophages, and dendritic cells, and has been shown to target naïve, resting CD4+ T cells to promote their proliferation and secretion of cytokines. The IL-12 p40 subunit is a secreted protein, which is synthesized with a signal peptide that is subsequently cleaved off to generate the mature form. It can homodimerize and the homodimeric form is shown to be about 25 to 50-fold more active than the monomer in competing with native IL-12 in receptor binding. IL-12 p40 can act as a growth factor for activated T and NK cells and can enhance the lytic activity of NK/lymphokine-activated killer cells and stimulate the production of IFN-gamma by resting peripheral blood mononuclear cells. It is also reported to associates with IL23A to form the IL-23 interleukin that functions in innate and adaptive immunity. (Ref.: Stallmach, A., et al. (2004). Gut. 53(3): 339-345).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.
Applications
ApplicationAnti-IL-12 p40, clone 11B10, Cat. No. MABF229, is a mouse monoclonal antibody that detects Interleukin-12 subunit beta and has been tested for use in Flow Cytometry and Immunohistochemistry (Paraffin).
Key Applications
  • Immunohistochemistry (Paraffin)
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: 1 µg from a representative lot detected IL-12 p40 in one million Jurkat cells.
Biological Information
ImmunogenHis-tagged full-length human recombinant Interleukin-12 subunit beta (IL12 p40).
Clone11B10
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 11B10 is a mouse monoclonal antibody that targets human Interleukin-12 p40 subunit.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • IL12B
  • NKSF2
Purification MethodProtein G purified
UniProt Number
Molecular Weight37.17 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human tonsil tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected IL-12 p40 in human tonsil tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2295-25UG 04061841276630