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MABS2234-25UG Anti-ICA 512/RESP18 Antibody, clone 1348-D29-1

Anti-ICA 512/RESP18, clone 1348-D29-1, Cat. No. MABS2234, is a mouse monoclonal antibody that detects ICA 512/RESP18 and is tested for use in Immunofluorescence and Western Blotting.

MABS2234-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABS2234-25UG
DescriptionAnti-ICA 512/RESP18 Antibody, clone 1348-D29-1
Alternate Names
  • Receptor-type tyrosine-protein phosphatase-like N
  • R-PTP-N
  • Islet cell antigen 512
  • ICA 512
  • Islet cell autoantigen 3
  • PTP IA-2
Background InformationReceptor-type tyrosine-protein phosphatase-like N (UniProt: P16849; also known as R-PTP-N, Islet cell antigen 512, ICA 512, Islet cell autoantigen 3, PTP IA-2) is encoded by the PTPRN (also known as ICA3, ICA512) gene (Gene ID: 5798) in human. ICA 512, a tyrosine phosphatase like intrinsic membrane protein, is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-34), which is subsequently cleaved off to produce the mature form that contains a lumenal domain (aa 35-575), a transmembrane domain (aa 576-600), and a cytoplasmic domain (aa 601-979). Its lumenal region contains the RESP18 homology domain that is localized in amino acids 35-131. The mature form can undergo cleavage to generate an N-terminal fragment (ICA 512-NTF), a transmembrane fragment (ICA 512-TMF), and a cleaved cytosolic fragment (ICA 512-CCF). ICA 512 expression is restricted to neuroendocrine cells and is detected in the pancreas, brain, and pituitary. It is involved in the biogenesis and turnover of insulin secretory granules (SGs) in pancreatic islet β-cells . It plays a role in insulin secretion in response to glucose. It is required for the accumulation of normal levels of insulin-containing vesicles and preventing their degradation. It plays a role in insulin secretion in response to glucose stimuli. It is also required for normal accumulation of secretory vesicles in hippocampus and pituitary. ICA 512-TMF is reported to regulate dynamics and exocytosis of insulin SGs. ICA 512-CCF is shown to translocate to the nucleus and promotes the expression of insulin and other granule-related genes. It enhances pancreatic b-cell proliferation by converging with signaling by STAT5B and STAT3. ICA 512-NTF containing the RESP18HD domain encodes for an intrinsically disordered region that acts as a condensing factor for the reversible aggregation of insulin and other β-cell proteins in a pH and zinc-regulated manner. (Ref.: Toledo, PL., et al. (2019). J. Biol. Chem. 294(21); 8564-8576; Mziaut, H., et al. (2008). Proc. Natl. Acad. Sci. USA. 105(2); 674-679; Trajkovski, M., et al. (2008). J. Biol. Chem. 283(48); 33719-33729).
References
Product Information
FormatPurified
Control
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PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-ICA 512/RESP18, clone 1348-D29-1, Cat. No. MABS2234, is a mouse monoclonal antibody that detects ICA 512/RESP18 and is tested for use in Immunofluorescence and Western Blotting.
Key Applications
  • Western Blotting
  • Immunofluorescence
Application NotesTested applications

Immunofluorescence Analysis: A representative lot detected ICA 512/RESP18 in Immunofluorescence applications (Toledo, P.L., et al. (2019). J Biol Chem. 294(21):8564-8576).

Western Blotting Analysis: A representative lot detected ICA 512/RESP18 in Western Blotting application (Toledo, P.L., et al. (2019). J Biol Chem. 294(21):8564-8576).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenGST-tagged recombinant protein fragment corresponding to the mature N-terminal fragment (without the signal peptide) of human ICA 512.
EpitopeN-terminal half
Clone1348-D29-1
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 1348-D29-1 is a mouse monoclonal antibody that detects ICA 512/RESP18. It targets an epitope within the RESP18 homology domain within the N-terminal fragment.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PTPRN
Purification MethodProtein G purified
UniProt Number
Molecular Weight~110-140 kDa observed; 105.85 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysate from EndoC βH1 cells.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected ICA 512/RESP18 in EndoC βH1 cells, but not in cells subjected to RNAi knockdown.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS2234-25UG 04065269049302