Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABD116 Anti-Human Oct-4 Antibody

This Anti-Human Oct-4 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of Human Oct-4.

Anti-Human Oct-4 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research, Stem Cell Research Research Sub-Category: Neural Stem Cells, Developmental Signaling Gene Symbol: POU5F1, Oct-3, MGC22487, Oct4, OTF4, OTF3, OCT3, OCT4, Oct-4 UniProt Number: Q01860
ABD116
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABD116
DescriptionAnti-Human Oct-4 Antibody
Alternate Names
  • POU domain,class 5, transcription factor 1
  • NF-A3
  • Octamer-binding protein 3
  • Oct-3
  • Octamer-binding protein 4
  • Oct-4
  • Octamer-binding transcription factor 3
  • OTF-3
Background InformationOctamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Human Oct-4 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of Human Oct-4.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:1000 dilution from a representative lot detected human Oct-4 in 10 µg of H9 human ES cells.
Biological Information
ImmunogenKLH-EDC-conjugated linear peptide corresponding to the unknown of Human Oct-4.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityReacts with human Oct-4 only, and detects the Oct-4A isoform. (Note: Please order Catalog No. MAB4419 for use with mouse embryonic stem cells).
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Chimpanzee, Goat based on 100% sequence homology. The clone reacts strongly with specific nuclear binding to human ES cells and cross-reacts weakly with mouse ES cells.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • POU5F1
  • Oct-3
  • MGC22487
  • Oct4
  • OTF4
  • OTF3
  • OCT3
  • OCT4
  • Oct-4
Purification MethodAntigen Affinity Purified
UniProt Number
Molecular Weight~40 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in H9 human ES cell lysate and mouse ES cells lysate.

Western Blotting Analysis: A 0.25 µg/mL dilution of this antibody detected human Oct-4 in 10 µg of H9 human ES cell lysate but not in 10 µg of mouse ES cells lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABD116 04055977109221