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MABF1975 Anti-Herpes Simplex Virus Glycoprotein D Antibody, clone LP14

Anti-Herpes Simplex Virus Glycoprotein D, clone LP14, Cat. No. MABF1975, is a mouse monoclonal antibody that detects Glycoprotein D of Herpes Simplex Virus and has been tested for use in Inhibition, Immunocytochemistry, and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: N/A UniProt Number: Q69091
MABF1975
100 µL  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
A Broad Range Of SpeciesInhibition, ICC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF1975
DescriptionAnti-Herpes Simplex Virus Glycoprotein D Antibody, clone LP14
Alternate Names
  • HSV glycoprotein D
Background InformationHerpes Simplex Virus envelope Glycoprotein D (UniProt: Q69091) is also known as gD. The envelope of herpes simplex virus (HSV) is complex and contains at least 10 virus-encoded glycoproteins, some of which mediate virus entry into the host cell. HSV virus enters the cell by first fusing its envelope with a membrane of the host cell. Five viral envelope glycoproteins are reported to be involved in this entry process. Binding of glycoprotein D of HSV to a cell surface receptor is required to trigger membrane fusion during entry into host cells. Glycoproteins C and B promote attachment by interacting with cell surface proteoglycans and then glycoprotein D binds to the herpes virus entry mediator (HVEM) receptor that initiates a process that ultimately leads to glycoprotein B-mediated membrane fusion. Glycoprotein D is synthesized with a signal peptide (aa 1-25) that is subsequently cleaved off. It is a homodimeric protein that contains two zinc-binding sites (aa 64 and 264). The ectodomain region of glycoprotein D contains three sites for the addition of N-linked oligosaccharides (N-CHO) and six cysteine residues arranged into three disulfide bonds. During virion morphogenesis, glycoprotein D probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs. (Ref.: Whitbeck JC et al. (1997). J. Virol. 71(8); p. 6083 6093; Nicola, AV et al. (1998). J Virol. 72(5): 3595-3601; Giovine, PD et al. (2011). PLos Pathog. 7(9); e1002277).
References
Product Information
FormatPurified
HS Code3002 15 90
PresentationPurified mouse monoclonal antibody IgG2a in PBS without azide.
Quality LevelMQ100
Applications
ApplicationAnti-Herpes Simplex Virus Glycoprotein D, clone LP14, Cat. No. MABF1975, is a mouse monoclonal antibody that detects Glycoprotein D of Herpes Simplex Virus and has been tested for use in Inhibition, Immunocytochemistry, and Western Blotting.
Key Applications
  • Inhibition
  • Immunocytochemistry
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:2,000 dilution from a representative lot detected Herpes Simplex Virus Glycoprotein D in lysates from VERO cells transfected with HSV-1.

Western Blotting Analysis: A representative lot detected Herpes Simplex Virus Glycoprotein D in Western Blotting applications (Lau, S.Y., et. al. (2015). Viruses. 7(3):915-38; Zenner, H.L., et. al. (2011). J Virol. 85(16):8012-21; Pawliczek, T., et. al. (2009). J Virol. 83(21):11254-64).

Western Blotting Analysis: A representative lot detected Herpes Simplex Virus Glycoprotein D in Western Blotting applications (Ren, Y., et. al. (2012). J Gen Virol. 93(Pt 2):319-29).

Immunocytochemistry Analysis: A representative lot detected Herpes Simplex Virus Glycoprotein D in Immunocytochemistry applications (Lau, S.Y., et. al. (2015). Viruses. 7(3):915-38).

Inhibition Analysis: A representative lot inhibited the fusion of cells infected with HSV-1. (Minson, A.C., et. al. (1986). J Gen Virol. 67(6); 1001-1013.
Biological Information
ImmunogenWhole Herpex Simplex Virus 2 (HSV-2).
CloneLP14
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone LP14 detects glycoprotein D of Herpes Simplex virus.
IsotypeIgG2aκ
Species Reactivity
  • A Broad Range Of Species
Species Reactivity NoteVirus.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • N/A
Purification MethodProtein G purified
UniProt Number
Molecular Weight~50 and 55 kDa observed; 43.35 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293T cell lysate transfected with HSV gD.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Herpes Simplex Virus Glycoprotein D in HEK293T cell lysate transfected with HSV gD.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF1975 04054839383557

Documentation

Anti-Herpes Simplex Virus Glycoprotein D Antibody, clone LP14 Certificates of Analysis

TitleLot Number
Anti-Herpes Simplex Virus Glycoprotein D, clone LP14 - 3604696 3604696
Anti-Herpes Simplex Virus Glycoprotein D, clone LP14 - 3714321 3714321
Anti-Herpes Simplex Virus Glycoprotein D, clone LP14 - 3768077 3768077
Anti-Herpes Simplex Virus Glycoprotein D, clone LP14 - 3999520 3999520
Anti-Herpes Simplex Virus Glycoprotein D, clone LP14 Monoclonal Antibody Q2959725
Anti-Herpes Simplex Virus Glycoprotein D, clone LP14 Monoclonal Antibody 3062184