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AB5378-50UL Anti-HCN2 Antibody

This Anti-HCN2 Antibody is validated for use in IH, WB for the detection of HCN2.

Anti-HCN2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: HCN2, BCNG-2, HAC-1, BCNG2 UniProt Number: Q9UL51
AB5378-50UL
50 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RIHC, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5378-50UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-HCN2 Antibody
Alternate Names
  • Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 2
  • BCNG2
  • HAC-1
References
Product Information
FormatAffinity Purified
HS Code3002 15 90
Control
  • CONTROL ANTIGEN:

    Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Quality LevelMQ100
Applications
ApplicationThis Anti-HCN2 Antibody is validated for use in IH, WB for the detection of HCN2.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesWestern blot: 1:200-1:1,000 using ECL on rat brain membranes.

Immunohistochemistry on rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenPurified peptide from human HCN2 (amino acids 147-161) (Accession CAB42602).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes HCN2 protein. The epitope is specific for HCN2 and is not present in any other known protein.
Species Reactivity
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HCN2
  • BCNG-2
  • HAC-1
  • BCNG2
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9UL51 # Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages (By similarity).
SIZE: 889 amino acids; 96950 Da
SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Heteromultimer with HCN1. Interacts with KCNE2 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Highly expressed throughout the brain. Detected at low levels in heart.
DOMAIN:SwissProt: Q9UL51 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
SIMILARITY: Belongs to the potassium channel HCN family. & Contains 1 cyclic nucleotide-binding domain.
MISCELLANEOUS: Inhibited by extracellular cesium ions.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB5378-50UL 04053252405938

Documentation

Anti-HCN2 Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-HCN2 Antibody Certificates of Analysis

TitleLot Number
RABBIT ANTI-HCN2 - 2555338 2555338
RABBIT ANTI-HCN2 - 3921935 3921935
RABBIT ANTI-HCN2 -2514890 2514890
RABBIT ANTI-HCN2 AFFINITY PURIFIED POLYCLONAL ANTIBODY 2925262
RABBIT ANTI-HCN2 AFFINITY PURIFIED POLYCLONAL ANTIBODY 3058477
RABBIT ANTI-HCN2 AFFINITY PURIFIED POLYCLONAL ANTIBODY 2937877
RABBIT ANTI-HCN2 AFFINITY PURIFIED POLYCLONAL ANTIBODY 2876895
RABBIT ANTI-HCN2 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2227103 2227103

References

Reference overviewPub Med ID
Regulation of the hyperpolarization-activated cationic current Ih in mouse hippocampal pyramidal neurones by vitronectin, a component of extracellular matrix
Vasilyev, Dmitry V and Barish, Michael E
J Physiol (Lond), 560:659-75 (2004)  2004

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