Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

AB5220 Anti-Growth Associated Protein-43 (GAP-43) Antibody

AB5220
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, R, Ch, BELISA, ICC, IHC, IP, WBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberAB5220
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Growth Associated Protein-43 (GAP-43) Antibody
Alternate Names
  • Neuromodulin
Background InformationGAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.
Product Information
FormatPurified
Control
  • Rat dorsal root ganglion tissue that has been subjected to a spinal nerve ligation, cultured neurons.
PresentationPurified rabbit polyclonal in buffer containing PBS with 0.01% sodium azide as a preservative.
Quality LevelMQ100
Applications
ApplicationAnti-Growth Associated Protein-43 (GAP-43) Antibody is an antibody against Growth Associated Protein-43 (GAP-43) for use in ELISA, IC, IH, IP & WB.
Key Applications
  • ELISA
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunohistochemistry:
1:1,000 dilution of a previous lot was used on 4% paraformaldehyde / PBS fixed or 3% Paraformaldehyde / 0.1% Gluteraldehyde / PBS fixed tissue sections.

Immunocytochemistry:
1:1,000 dilution of a previous lot was used on Hippocampal neurons in culture and PC12 cells stimulated with NGF.

Immunoprecipitation:
1:100 dilution of a previous lot was used.

ELISA:
1:2,000 dilution of a previous lot was used.
Biological Information
ImmunogenRecombinant rat GAP-43 (complete sequence).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes Growth Associated Protein-43 (GAP-43).
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Chicken
  • Bovine
Species Reactivity NoteReacts to a high degree with mouse. Reacts to a lesser degree with rat, human, bovine and chicken. Other species have not been tested.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This encoded protein is considered a crucial component of an effective regenerative response in the nervous system.
Gene Symbol
  • GAP43
  • PP46
  • B-50
Purification MethodAmmonium Sulfate Precipitation
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P17677 # This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons.
SIZE: 238 amino acids; 24803 Da
SUBUNIT: Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Note=Cytoplasmic surface of growth cone and synaptic plasma membranes.
PTM: Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
SIMILARITY: SwissProt: P17677 ## Belongs to the neuromodulin family. & Contains 1 IQ domain.
Molecular Weight23.6 kDa
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on Mouse brain lysates.

Western Blot Analysis: 1:500 dilution of this lot detected Growth Associated Protein 43 on 10 μg of Mouse brain lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for up to 6 months at -20ºC in undiluted aliquots from date of receipt.
Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalogue Number GTIN
AB5220 04053252676963