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MABF2069 Anti-Glucuronoxylomannan (GXM) Antibody, clone 18B7

Anti-Glucuronoxylomannan (GXM), clone 18B7, Cat. No. MABF2069, is a mouse monoclonal antibody for detection of Cryptococcus neoformans Glucuronoxylomannan and has been tested for use in and ELISA, Immunodepletion, Immunocytochemistry, Immunofluorescence, and Immunohistochemistry (Paraffin),

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: N/A
MABF2069
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MELISA, Immunodepletion, ICC, IF, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF2069
DescriptionAnti-Glucuronoxylomannan (GXM) Antibody, clone 18B7
Alternate Names
  • GXM
Background InformationCryptococcus neoformans is an encapsulated yeast-like fungus that is reported to causes systemic infections, including fatal meningoencephalitis. Glucuronoxylomannan (GXM) is the principal constituent of the Cryptococcus neoformans capsule that modulates the inflammatory response of human monocytes in vitro. The basic structural unit of GXM is a tri-mannose repeat with a glucuronic acid residue in the first mannose. Its structure is shown to be further modified in individual strains by the addition of xylose substitutions on the mannan backbone. Six triads known as M1-M2 are shown to be present in various proportions in GXM from the various serotypes and M2 is reported to be the most common triad in serotype A GXM. Soluble GXM serves as an important virulence factor and contributes to the pathogenesis of infection, and high levels of GXM have been found in serum of patients with cryptococcosis. Clone 18B7 displays significant activity against GXM and is reported act by possibly hydrolyzing the oligosaccharide substrate. This antibody acts as a potent opsonin for Cryptococcus neoforman by increasing its phagocytosis. This antibody may also reduce GXM levels by interfering with the release of polysaccharide from the capsule. Administration of this antibody to mice is shown to rapidly clear serum Cryptococcal antigen. (Ref.: Bowen, A., et al. (2017). J. Biol. Chem. 292(2); 417-434; Martinez, LR., et al. (2004). Infect. Immun. 72(6); 3674 3679; Tissi, L., et al. (2004). Infect. Immun. 72(11); 6367-6372; Devi, SJN et al. (1991). Infect. Immun. 59(10); 3700-3707).
References
Product Information
FormatPurified
HS Code3002 15 90
PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
Quality LevelMQ100
Applications
ApplicationAnti-Glucuronoxylomannan (GXM), clone 18B7, Cat. No. MABF2069, is a mouse monoclonal antibody for detection of Cryptococcus neoformans Glucuronoxylomannan and has been tested for use in and ELISA, Immunodepletion, Immunocytochemistry, Immunofluorescence, and Immunohistochemistry (Paraffin),
Key Applications
  • ELISA
  • Immunodepletion
  • Immunocytochemistry
  • Immunofluorescence
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A representative lot detected Glucuronoxylomannan (GXM) in Immunohistochemistry applications (Lendvai, N., et. al. (1999). J Infect Dis. 180(3):791-801; Casadevall, A., et. al. (1998). Antimicrob Agents Chemother. 42(6):1437-46).

Immunodepletion Analysis: A representative lot immunodepleted Glucuronoxylomannan (GXM) in Immunodepletion applications (Lendvai, N., et. al. (1999). J Infect Dis. 180(3):791-801; Casadevall, A., et. al. (1998). Antimicrob Agents Chemother. 42(6):1437-46; Martinez, L.R., et. al. (2004). Infect Immun. 72(6):3674-9; Bowen, A., et. al. (2017). J Biol Chem. 292(2):417-434).

Immunofluorescence Analysis: A representative lot detected Glucuronoxylomannan (GXM) in Immunofluorescence applications (Casadevall, A., et. al. (1998). Antimicrob Agents Chemother. 42(6):1437-46; Bowen, A., et. al. (2017). J Biol Chem. 292(2):417-434).

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Glucuronoxylomannan (GXM) in heat killed cryptococcus cells.

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Glucuronoxylomannan (GXM) in S. neoformans-infected mouse spleen, mouse lung, mouse liver, and mouse brain tissues.

ELISA Analysis: A representative lot detected Glucuronoxylomannan (GXM) in ELISA applications (Casadevall, A., et. al. (1998). Antimicrob Agents Chemother. 42(6):1437-46; Martinez, L.R., et. al. (2004). Infect Immun. 72(6):3674-9; Bowen, A., et. al. (2017). J Biol Chem. 292(2):417-434).
Biological Information
ImmunogenCryptococcus neoformans serotype A glucuronoxylomannan (GXM)-Tetanus Toxoid conjugate vaccine.
Clone18B7
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 18B7 is a mouse monoclonal antibody that binds to Glucuronoxylomannan (GXM) from Cryptococcus neoformans.
IsotypeIgG1κ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • N/A
Purification MethodProtein G purified
Molecular WeightN/A
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2069 04054839415258

Documentation

Anti-Glucuronoxylomannan (GXM) Antibody, clone 18B7 SDS

Title

Safety Data Sheet (SDS) 

Anti-Glucuronoxylomannan (GXM) Antibody, clone 18B7 Certificates of Analysis

TitleLot Number
Anti-Glucuronoxylomannan (GXM), clone 18B7 - 3160112 3160112
Anti-Glucuronoxylomannan (GXM), clone 18B7 - 3302958 3302958
Anti-Glucuronoxylomannan (GXM), clone 18B7 - 3506491 3506491
Anti-Glucuronoxylomannan (GXM), clone 18B7 - 3717006 3717006
Anti-Glucuronoxylomannan (GXM), clone 18B7 - 3735796 3735796
Anti-Glucuronoxylomannan (GXM), clone 18B7 - 3789393 3789393
Anti-Glucuronoxylomannan (GXM), clone 18B7 - 3800108 3800108
Anti-Glucuronoxylomannan (GXM), clone 18B7 - 3929943 3929943
Anti-Glucuronoxylomannan (GXM), clone 18B7 - 4001929 4001929
Anti-Glucuronoxylomannan (GXM), clone 18B7 - 4013866 4013866
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