Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Anti-Giα-3-Subunit, C-Terminal (345-354), rabbit polyclonal, recognizes Giα-3 subunit. Does not cross-react with Gsα & Goα subunits. Validated for use in WB & IP.
More>>Anti-Giα-3-Subunit, C-Terminal (345-354), rabbit polyclonal, recognizes Giα-3 subunit. Does not cross-react with Gsα & Goα subunits. Validated for use in WB & IP. Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Immunoblotting (1:1000) Immunoprecipitation (see comments)
Application Comments
The specificity was confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, GIα-2, Giα-3, and Goα. The original titer of the serum was 1:10,000. Suitable for immunoblotting and immunoprecipitation. Does not cross-react with Gsα and Goα. Antibody partially cross-reacts with Giα-1 and GIα-2. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
a synthetic peptide (CKNNLKECGLY) corresponding to amino acids at the C-terminus of mammalian Giα-3, conjugated to KLH
Host
Rabbit
Isotype
IgG
Species Reactivity
Mammals
Antibody Type
Polyclonal Antibody
Storage and Shipping Information
Ship Code
Dry Ice Only
Toxicity
Standard Handling
Storage
≤ -70°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Anti-Giα-3-Subunit, C-Terminal (345-354) Rabbit pAb Certificates of Analysis
Title
Lot Number
371729
References
Reference overview
Kumar, R., et al. 1994. J. Mol. Cell. Cardiol. 26, 1537. Raymond, J.R., et al. 1993. Biochemistry32, 11064. Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215. Jones, D.T., and Reed, R.R. 1987. J. Biol. Chem.262, 14,241.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
02-August-2007 RFH
Application
Immunoblotting (1:1000) Immunoprecipitation (see comments)
Description
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the Giα-3 subunit protein.
Host
Rabbit
Immunogen
a synthetic peptide (CKNNLKECGLY) corresponding to amino acids at the C-terminus of mammalian Giα-3, conjugated to KLH
Isotype
IgG
Species
mammalian
Form
Liquid
Formulation
In 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
Preservative
None
Comments
The specificity was confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, GIα-2, Giα-3, and Goα. The original titer of the serum was 1:10,000. Suitable for immunoblotting and immunoprecipitation. Does not cross-react with Gsα and Goα. Antibody partially cross-reacts with Giα-1 and GIα-2. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw ≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Toxicity
Standard Handling
References
Kumar, R., et al. 1994. J. Mol. Cell. Cardiol. 26, 1537. Raymond, J.R., et al. 1993. Biochemistry32, 11064. Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215. Jones, D.T., and Reed, R.R. 1987. J. Biol. Chem.262, 14,241.
