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ABC299 Anti-FFAR2 Antibody/GPR43

Anti-FFAR2/GPR43, Cat. No. ABC299, is a rabbit polyclonal antibody that detects FFAR2/GPR43 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-FFAR2 Antibody/GPR43 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: FFAR2, GPR43 UniProt Number: O15552
ABC299
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABC299
DescriptionAnti-FFAR2 Antibody/GPR43
Alternate Names
  • Free fatty acid receptor 2
  • G-protein coupled receptor 43
  • FFAR2/GPR43
Background InformationFree fatty acid receptor 2 (UniProt: O15552; also known as G-protein coupled receptor 43) is encoded by the FFAR2 (also known as FFA2, GPCR43, GPR43) gene (Gene ID: 2867) in human. FFAR2 is a multi-pass membrane glycoprotein with four extracellular domains, seven transmembrane domains, and four cytoplasmic domains. It is expressed at relatively high levels in peripheral blood leukocytes and at lower levels in the spleen. It is a G-protein-coupled receptor that is activated by short chain fatty acids (SCFAs) and plays a role in the regulation of whole-body energy homeostasis and in intestinal immunity. In omnivorous mammals, SCFAs containing less than 6 carbons, such as acetate, propionate, and butyrate, produced by the gut microbiome, are the most potent activators. These SCFAs not only serve as a source of energy, but also act as signaling molecules. The activation of this G-protein-coupled receptor leads to the formation of inositol 1,4,5-trisphosphate leading to the mobilization of intracellular calcium and phosphorylation of ERK1 and ERK2. It also plays a role in glucose homeostasis by regulating the secretion of GLP-1. It is reported to regulate whole-body energy homeostasis through adipogenesis regulating both differentiation and lipid storage of adipocytes. In parallel, FFAR2 also mediates the activation of the inflammatory chemokines and cytokines. FFAR2 is also involved in maintaining healthy gut microbiome and suppress intestinal carcinogenesis and its down modulation has been linked to colon cancers. (Ref.: Sivaprakasam, S., et al. (2016). Oncogenesis. 5; 238).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-FFAR2/GPR43, Cat. No. ABC299, is a rabbit polyclonal antibody that detects FFAR2/GPR43 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesTested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected FFAR2/GPR43 in mouse kidney and mouse liver tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 54 amino acids from the C-terminal region of human Free fatty acid receptor 2 (FFAR2).
EpitopeCytoplasmic domain
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects Free fatty acid receptor 2 (FFAR2). It targets an epitope within the C-terminal region.
Species Reactivity
  • Mouse
Species Reactivity NoteMouse. Predicted to react with Human, Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • FFAR2
  • GPR43
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~50 kDa observed; 37.14 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in RAW264.7 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected FFAR2/GPR43 in RAW264.7 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABC299 04053252985188

Documentation

Anti-FFAR2 Antibody/GPR43 SDS

Title

Safety Data Sheet (SDS) 

Anti-FFAR2 Antibody/GPR43 Certificates of Analysis

TitleLot Number
Anti-FFAR2 Antibody/GPR43 - 3694826 3694826
Anti-FFAR2 Antibody/GPR43 - 3715715 3715715
Anti-FFAR2 Antibody/GPR43 - 3739772 3739772
Anti-FFAR2/GPR43 - Q2444408 Q2444408
Anti-FFAR2/GPR43 - 3218239 3218239
Anti-FFAR2/GPR43 - 3323154 3323154
Anti-FFAR2/GPR43 - 3458523 3458523
Anti-FFAR2/GPR43 - 3881001 3881001
Anti-FFAR2/GPR43 - 4061134 4061134
Anti-FFAR2/GPR43 - 4069778 4069778
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