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MABS1286-25UG Anti-EPO-R Antibody, clone BCO-3H2

Anti-EPO-R, clone BCO-3H2, Cat. No. MABS1286, is a rat monoclonal antibody that detects erythropoietin receptor and has been tested for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Anti-EPO-R Antibody, clone BCO-3H2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: EPOR UniProt Number: P19235
MABS1286-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIF, IHC, IP, WBRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1286-25UG
DescriptionAnti-EPO-R Antibody, clone BCO-3H2
Alternate Names
  • Erythropoietin receptor
Background InformationErythropoietin receptor (UniProt: P19235; also known as EPO-R) is encoded by the EPOR gene (Gene ID: 2057) in human. EPO-R is a single-pass type 1 membrane protein that mediates erythropoietin-induced erythroblast proliferation and differentiation. It is synthesized with a signal peptide (aa 1-24) that is cleaved to produce the mature form. Upon EPO stimulation, EPO-R homodimerizes and gets phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. The tyrosine-phosphorylated form interacts with several SH2 domain-containing proteins. The EPO-R is expressed in erythroid cells and erythroid progenitor cells. Three isoforms of EPO-R has been described that are produced by alternate splicing. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early-stage erythroid progenitor cells. Mutation in EPOR gene are linked to familial erythrocytosis that is characterized by elevated serum red blood cell mass, hemoglobin and hematocrit, and increased sensitivity of erythroid progenitors to erythropoietin.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2b in PBS without azide.
Applications
ApplicationAnti-EPO-R, clone BCO-3H2, Cat. No. MABS1286, is a rat monoclonal antibody that detects erythropoietin receptor and has been tested for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A representative lot detected EPO-R in Immunohistochemistry applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).

Immunoprecipitation Analysis: A representative lot detected EPO-R in Immunoprecipitation applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).

Immunofluorescence Analysis: A representative lot detected EPO-R in Immunofluorescence applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).

Western Blotting Analysis: A representative lot detected EPO-R in Western Blotting applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 17 amino acids from the human erythropoietin receptor.
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone BCO-3H2 is a rat monoclonal antibody that detects erythopoietin receptor in human cells. It targets an epitope with in 17 amino acids from the C-terminal region.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • EPOR
Purification MethodProtein G purified
UniProt Number
Molecular Weight~58 kDa observed; 55.07 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in UT-7 cell lysates.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected EPO-R in 10 µg of UT-7 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1286-25UG 04054839497070