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MABS1915-25UG Anti-EGFRvIII Antibody, clone DH8.3

Anti–EGFRvIII, clone DH8.3 Antibody, Cat. No. MABS1915-25UG, validated for use in Western Blotting, ELISA, Immunohistochemistry, Flow Cytometry and Immunoprecipitation.

Anti-EGFRvIII Antibody, clone DH8.3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: EGFR, ERBB, ERBB1, HER1 UniProt Number: P00533
MABS1915-25UG
25 μg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ELISA, IHC, FC, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1915-25UG
DescriptionAnti-EGFRvIII Antibody, clone DH8.3
Alternate Names
  • Proto-oncogene c-ErbB-1
  • Receptor tyrosine-protein kinase erbB-1
  • Epidermal growth facttor receptor vIII
Background InformationEpidermal growth factor receptor (UniProt: P00533; also known as EC:2.7.10.1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1) is encoded by the EGFR (also known as ERBB, ERBB1, HER1) gene (Gene ID: 1956) in human. EGFR is a single-pass type 1 membrane protein that is ubiquitously expressed. It is synthesized with a 24 amino acids signal peptide that is subsequently cleaved. It contains an extracellular domain (aa 25-645) a transmembrane domain (aa 646-668), and a cytoplasmic domain (aa 669-1210). Upon binding of EGF, the receptor is phosphorylated and recruits adapter proteins to turn on downstream signaling cascades. EGFR is commonly overexpressed and is mutated in many human malignancies and is often associated with aggressive phenotypes. The most common truncated EGFR is the variant III EGFR deletion mutant (EGFRvIII), containing an inframe deletion of exons 2 7 (801 bp) from the extracellular domain. EGFRvIII mutation is reported in about 5% of human lung squamous cell carcinoma (SCC). It has also been suggested that EGFRvIII potentially plays an essential role in the human NSCLC tumor initiation and maintenance. Drugs such as Gefitinib, erlotnib, and HKI-272 are shown to inhibit the growth of EGFRvIII transformed cells and tumor growth. (Ref.: Ji, H., et al. (2006). Proc. Natl. Acad. Sci. USA 103(20); 7817-7822).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti–EGFRvIII, clone DH8.3 Antibody, Cat. No. MABS1915-25UG, validated for use in Western Blotting, ELISA, Immunohistochemistry, Flow Cytometry and Immunoprecipitation.
Key Applications
  • Western Blotting
  • ELISA
  • Immunohistochemistry
  • Flow Cytometry
  • Immunoprecipitation
Application NotesWestern Blotting Analysis: A representative lot detected EGFRvIII in Western Blotting applications (Lammering, G., et. al. (2004). Clin Cancer Res. 10(19):6732-43; Nishikawa, R., et. al. (2004). Brain Tumor Pathol. 21(2):53-6; Hills, D., et. al. (1995). Int J Cancer. 63(4):537-43).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to EGFRvIII (EGFR variant III) (LEEKKGNYVVTDHC) sequence.
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone DH8.3 detects EGFR variant vIII a truncated form of EGFR, present in human cancer cells.
IsotypeIgG1
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • EGFR
  • ERBB
  • ERBB1
  • HER1
Purification MethodProtein G purified
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in U87MG.∆EGFR cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1915-25UG 04054839420986