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MABS1173 Anti-E3 ubiquitin-protein ligase CBL-C Antibody, clone 10F4.2

This Anti-E3 ubiquitin-protein ligase CBL-C Antibody, clone 10F4.2 is validated for use in Western Blotting for the detection of E3 ubiquitin-protein ligase CBL-C.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Ubiquitin & Ubiquitin Metabolism Gene Symbol: CBLC, CBL3, RNF57 UniProt Number: Q9ULV8
MABS1173
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1173
DescriptionAnti-E3 ubiquitin-protein ligase CBL-C Antibody, clone 10F4.2
Alternate Names
  • E3 ubiquitin-protein ligase CBL-C
  • RING finger protein 57
  • SH3-binding protein CBL-3
  • SH3-binding protein CBL-C
  • Signal transduction protein CBL-C
Background InformationE3 ubiquitin-protein ligase CBL-C (UniProt Q9ULV8; also known as RING finger protein 57, SH3-binding protein CBL-3, SH3-binding protein CBL-C, Signal transduction protein CBL-C) is encoded by the CBLC (also known as CBL3, RNF57) gene (Gene ID 23624) in human. CBLC belongs to the CBL family of E3 ubiquitin ligases, members of which also include CBL (a.k.a. c-CBL) and CBL-B. Similar to CBL and CBL-B, CBL-C binds SH3 (Src homology 3) domain-containing proto-oncogenic tyrosine kinases such as EGFR, Lyn, Crk, Src and Ret. CBL-C is recruited to EGFR upon EGF stimulation, leading to CBL-C phosphorylation by EGFR and subsequent downstream MAP kinase signalling. In addition to mediating oncoprotein Ret ubiquitination for proteasomal degradation, CBL-C is shown to form a complex with HIC5 (Hydrogen peroxide-inducible clone 5) and the heat shock protein HSP27 that are necessary for the ubiquitination of NOX4. Unlike the other CBL family members, CBL-C expression appears to be restricted to epithelial tissues. Transgenic expression of CBL-C in mouse mammary gland causes impairment of cell proliferation, suggesting that CBL-C might have a growth-inhibitory role. CBL-C contains an N-terminal Cbl-type phosphotyrosine-binding (Cbl-PTB) domain (a.a. 7-321) that mediates SH3 domain interaction and a C-terminal RET-interacting region (a.a. 351-474) harboring the catalytic ring finger domain (a.a. 351-390) that mediates interaction with E2 ubiquitin-conjugating enzyme. The PTB domain, also known as tyrosine kinase-binding (TKB) domain, is subdivided into a four-helix bundle or 4H domain (a.a. 7-145), a calcium-binding EF hand-like domain (a.a. 146-218), and a SH2-like domain (a.a. 219-321).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-E3 ubiquitin-protein ligase CBL-C Antibody, clone 10F4.2 is validated for use in Western Blotting for the detection of E3 ubiquitin-protein ligase CBL-C.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST-tagged recombinant fragment from the RET-interacting region of human CBL-C.
EpitopeRET-interacting region.
Clone10F4.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 10F4.2 targets an epitope in the RET-interacting region present in both spliced isoforms of human CBL-C reported by UniProt (Q9ULV8).
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CBLC
  • CBL3
  • RNF57
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~50 kDa observed. 52.46 kDa (isoform 1; Long) and 47.42 kDa (isoform 2; Short) calculated. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HT-29 human colon cancer cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected CBL-C in 10 µg of HT-29 human colon cancer cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1173 04055977352979

Documentation

Anti-E3 ubiquitin-protein ligase CBL-C Antibody, clone 10F4.2 SDS

Title

Safety Data Sheet (SDS) 

Anti-E3 ubiquitin-protein ligase CBL-C Antibody, clone 10F4.2 Certificates of Analysis

TitleLot Number
Anti-E3 ubiquitin-protein ligase CBL-C, clone 10F4.2 - 3290410 3290410
Anti-E3 ubiquitin-protein ligase CBL-C, clone 10F4.2 - 3330357 3330357
Anti-E3 ubiquitin-protein ligase CBL-C, clone 10F4.2 - 3468666 3468666
Anti-E3 ubiquitin-protein ligase CBL-C, clone 10F4.2 - 3745497 3745497
Anti-E3 ubiquitin-protein ligase CBL-C, clone 10F4.2 -Q2690249 Q2690249