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ABF1159 Anti-DEFA1

This rabbit polyclonal Anti-DEFA1 Antibody, Cat. No. ABF1159 is validated for use in Flow Cytometry and Western Blotting for the detection of HP 1-56.

Anti-DEFA1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: DEFA1, DEF1, DEFA2, MRS, DEFA1B UniProt Number: P59665
ABF1159
50 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, FCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABF1159
DescriptionAnti-DEFA1
Alternate Names
  • HP 1-56
  • Neutrophil defensin HP 1-56 chain
Background InformationThe human DEFA1 gene (also known as DEF1, DEFA2, MRS, DEFA1B; Gene ID 1667) encodes a 94-amino acid precursor protein (UniProt P59665) with an N-terminal signal peptide (a.a. 1-19) and propeptide (a.a. 20-38) sequence. Posttranslational processing by proteolytic cleavages generates three neutrophil granule peptides, HP 1-56 (a.a. 39-94), neutrophil defensin 1 (HNP-1, HP-1, HP1; a.a. 65-94), and neutrophil defensin 2 (HNP-2, HP-2, HP2; a.a. 66-94). These peptides exhibit several biological activities including the non-oxidative destruction of ingested microorganisms, the inhibition of adrenocorticotropin-stimulated glucocorticoids synthesis, monocyte chemotaxis, the non-cytolytic inhibition of thymidine incorporation in HL-60 promyelocyte-like cells and the stimulation of nifedipine-sensitive calcium channels. The richest source of HP1-56 relative to HP-1 is found in fetal lung tissue. In addition, HP1-56 is found in the HL-60 promyelocytic cell line and in some leukemias, but not in normal mature circulating neutrophils.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis rabbit polyclonal Anti-DEFA1 Antibody, Cat. No. ABF1159 is validated for use in Flow Cytometry and Western Blotting for the detection of HP 1-56.
Key Applications
  • Western Blotting
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: 0.1 µL from a representative lot detected HP 1-56 immunoreactivity in one million 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized HL-60 human promyelocytic leukemia cells.
Biological Information
ImmunogenKLH-conjuated linear peptide corresponding to an internal sequence of human HP 1-56.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody targets a region present in prepro-, pro-, and mature HP 1-56 (a.a. 1-94, 20-94, and 39-94, respectively). Target region is absent from neutrophil defensin 1 (HNP-1, HP-1, HP1; a.a. 65-94) and neutrophil defensin 2 (HNP-2, HP-2, HP2; a.a. 66-94).
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Rhesus Macaque based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • DEFA1
  • DEF1
  • DEFA2
  • MRS
  • DEFA1B
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~10 kDa observed. 10.20/8.238/6.306 kDa (prepro-/pro-/mature HP 1-56) calculted. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HL-60 cell lysate.

Western Blotting Analysis: A 1:5000 dilution of this antibody detected HP 1-56 in 10 µg of HL-60 human promyelocytic leukemia cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABF1159 04054839056314